Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase

C. R LIN; M. S KAPILOFF; S DURGERIAN; K TATEMOTO; A. F RUSSO; Phyllis Hanson; H SCHULMAN; M. G ROSENFELD

doi:10.1073/pnas.84.16.5962

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Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase

Journal article

Open access

Peer reviewed

Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase

C. R LIN, M. S KAPILOFF, S DURGERIAN, K TATEMOTO, A. F RUSSO, Phyllis Hanson, H SCHULMAN and M. G ROSENFELD

Proceedings of the National Academy of Sciences - PNAS, Vol.84(16), pp.5962-5966

1987

DOI: 10.1073/pnas.84.16.5962

PMID: 3475713

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Abstract

A calcium/calmodulin-dependent protein kinase type II (CaM-K) alpha-subunit cDNA has been cloned from rat brain. This enzyme is encoded by a 5.1-kilobase mRNA expressed exclusively in the brain. Hybridization histochemistry reveals that the CaM-K mRNA expression corresponds to the distribution of the immunoreactive alpha-subunit protein, suggesting that the high enzyme levels in specific brain areas reflect regional differences in gene expression. The sequence of CaM-K alpha-subunit cDNA indicates a 478-amino acid (54-kDa) protein with three functional domains. The domain organization suggests a structural model for calcium/calmodulin-dependent and independent states that might subserve short- and long-term responses to transient stimuli.

Biotechnology

Genetic Engineering

Molecular Cloning

Fundamental and applied biological sciences. Psychology

Methods. Procedures. Technologies

Genetic technics

Biological and medical sciences

Details

Title: Subtitle: Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase
Creators: C. R LIN - Univ. California, school medicine, eukaryotic regulatory biology program, San Diego La Jolla CA 92093, United States
M. S KAPILOFF - Univ. California, school medicine, eukaryotic regulatory biology program, San Diego La Jolla CA 92093, United States
S DURGERIAN - Univ. California, school medicine, eukaryotic regulatory biology program, San Diego La Jolla CA 92093, United States
K TATEMOTO - Univ. California, school medicine, eukaryotic regulatory biology program, San Diego La Jolla CA 92093, United States
A. F RUSSO - Univ. California, school medicine, eukaryotic regulatory biology program, San Diego La Jolla CA 92093, United States
Phyllis Hanson - Stanford University School of Medicine
H SCHULMAN - Univ. California, school medicine, eukaryotic regulatory biology program, San Diego La Jolla CA 92093, United States
M. G ROSENFELD - Univ. California, school medicine, eukaryotic regulatory biology program, San Diego La Jolla CA 92093, United States
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.84(16), pp.5962-5966
DOI: 10.1073/pnas.84.16.5962
PMID: 3475713
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences; Washington, DC
Language: English
Date published: 1987
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Craniofacial Anomalies Research Center
Record Identifier: 9984020872002771

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