Molecular cloning of a human melanoma-associated chondroitin sulfate proteoglycan

G Pluschke; M Vanek; Amanda Evans; T Dittmar; Peter Schmid; P Itin; Edward J Filardo; R A Reisfeld

doi:10.1073/pnas.93.18.9710

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Molecular cloning of a human melanoma-associated chondroitin sulfate proteoglycan

Journal article

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Molecular cloning of a human melanoma-associated chondroitin sulfate proteoglycan

G Pluschke, M Vanek, Amanda Evans, T Dittmar, Peter Schmid, P Itin, Edward J Filardo and R A Reisfeld

Proceedings of the National Academy of Sciences - PNAS, Vol.93(18), pp.9710-9715

09/03/1996

DOI: 10.1073/pnas.93.18.9710

PMCID: PMC38494

PMID: 8790396

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Abstract

A human melanoma-associated chondroitin sulfate proteoglycan (MCSP), recognized by mAb 9.2.27, plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. We report here the molecular cloning and nucleotide sequencing of cDNA encoding the entire core protein of human MCSP and provide its deduced amino acid sequence. This core protein contains an open reading frame of 2322 aa, encompassing a large extracellular domain, a hydrophobic transmembrane region, and a relatively short cytoplasmic tail. Northern blot analysis indicated that MCSP cDNA probes detect a single 8.0-kb RNA species expressed in human melanoma cell lines. In situ hybridization experiments with a segment of the MCSP coding sequence localized MCSP mRNA in biopsies prepared from melanoma skin metastases. Multiple human Northern blots with an MCSP-specific probe revealed a strong hybridization signal only with melanoma cells and not with other human cancer cells or a variety of human fetal and adult tissues. These data indicate that MCSP represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. The availability of cDNAs encoding MCSP should facilitate studies designed to establish correlations between structure and function of this molecule and help to establish its role in the progression of human malignant melanoma.

Details

Title: Subtitle: Molecular cloning of a human melanoma-associated chondroitin sulfate proteoglycan
Creators: G Pluschke - Pharmaceuticals Research Division, CIBA-Geigy, Ltd., Basel, Switzerland
M Vanek - Pharmaceuticals Research Division, CIBA-Geigy, Ltd., Basel, Switzerland
Amanda Evans - Pharmaceuticals Research Division, CIBA-Geigy, Ltd., Basel, Switzerland
T Dittmar - Pharmaceuticals Research Division, CIBA-Geigy, Ltd., Basel, Switzerland
Peter Schmid - Pharmaceuticals Research Division, CIBA-Geigy, Ltd., Basel, Switzerland
P Itin - Pharmaceuticals Research Division, CIBA-Geigy, Ltd., Basel, Switzerland
Edward J Filardo - Pharmaceuticals Research Division, CIBA-Geigy, Ltd., Basel, Switzerland
R A Reisfeld - Pharmaceuticals Research Division, CIBA-Geigy, Ltd., Basel, Switzerland
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.93(18), pp.9710-9715
DOI: 10.1073/pnas.93.18.9710
PMID: 8790396
PMCID: PMC38494
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Language: English
Date published: 09/03/1996
Academic Unit: Pharmaceutical Sciences and Experimental Therapeutics; Cardiovascular Medicine; Surgery; Internal Medicine
Record Identifier: 9984051985202771

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