Journal article
Molecular cloning of mtp70, a mitochondrial member of the hsp70 family
Molecular and cellular biology, Vol.9(11), pp.5163-5168
11/1989
DOI: 10.1128/mcb.9.11.5163-5168.1989
PMCID: PMC363668
PMID: 2689873
Abstract
We have isolated a gene from the protozoan parasite Trypanosoma cruzi that encodes a previously unidentified member of the 70-kilodalton heat shock protein (hsp70) family. Among all the eucaryotic hsp70 proteins described to date, this trypanosome protein, mtp70, is uniquely related in sequence and structure to the hsp70 of Escherichia coli, DnaK, which functions in the initiation of DNA replication. This relationship to DnaK is especially relevant in view of the intracellular location of the protein. Within the trypanosome, mtp70 is located in the mitochondrion, where it associates with kinetoplast DNA (kDNA), the unusual mitochondrial DNA that distinguishes this order of protozoa. Moreover, mtp70 is located in the specific region of the kinetoplast in which kDNA replication occurs. In view of the known functions of DnaK, the localization of mtp70 to the site of kDNA replication suggests that mtp70 may participate in eucaryotic mitochondrial DNA replication in a manner analogous to that of DnaK in E. coli.
Details
- Title: Subtitle
- Molecular cloning of mtp70, a mitochondrial member of the hsp70 family
- Creators
- David M Engman - University of IowaLouis V Kirchhoff - University of IowaJohn E Donelson - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular biology, Vol.9(11), pp.5163-5168
- DOI
- 10.1128/mcb.9.11.5163-5168.1989
- PMID
- 2689873
- PMCID
- PMC363668
- ISSN
- 0270-7306
- eISSN
- 1098-5549
- Language
- English
- Date published
- 11/1989
- Academic Unit
- Psychiatry; Infectious Diseases; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984280866102771
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