Journal article
Molecular concordance of methicillin-resistant Staphylococcus aureus isolates from healthcare workers and patients
Infection control and hospital epidemiology, Vol.44(4), pp.578-588
04/01/2023
DOI: 10.1017/ice.2022.159
PMID: 36177884
Abstract
Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant nosocomial pathogen in the ICU. MRSA contamination of healthcare personnel (HCP) gloves and gowns after providing care to patients with MRSA occurs at a rate of 14%-16% in the ICU setting. Little is known about whether the MRSA isolates identified on HCP gown and gloves following patient care activities are the same as MRSA isolates identified as colonizing or infecting the patient. Methods: From a multisite cohort of 388 independent patient MRSA isolates and their corresponding HCP gown and glove isolates, we selected 91 isolates pairs using a probability to proportion size (PPS) sampling method. To determine whether the patient and HCP gown or gloves isolates were genetically similar, we used 5 comparative genomic typing methods: phylogenetic analysis, spa typing, multilocus sequence typing (MLST), large-scale BLAST score ratio (LSBSR), and single-nucleotide variant (SNV) analysis. Results: We identified that 56 (61.5%) of isolate pairs were genetically similar at least by 4 of the methods. Comparably, the spa typing and the LSBSR analyses revealed that >75% of the examined isolate pairs were concordant, with the thresholds established for each analysis. Conclusions: Many of the patient MRSA isolates were genetically similar to those on the HCP gown or gloves following a patient care activity. This finding indicates that the patient is often the primary source of the MRSA isolates transmitted to the HCP, which can potentially be spread to other patients or hospital settings through HCP vectors. These results have important implications because they provide additional evidence for hospitals considering ending the use of contact precautions (gloves and gowns) for MRSA patients.
Details
- Title: Subtitle
- Molecular concordance of methicillin-resistant Staphylococcus aureus isolates from healthcare workers and patients
- Creators
- Timileyin Y. Adediran - University of Maryland, BaltimoreStephanie Hitchcock - University of Maryland, BaltimoreJ. Kristie Johnson - University of Maryland, BaltimoreO. Colin Stine - University of Maryland, BaltimoreSurbhi Leekha - University of Maryland, BaltimoreKerri A. Thom - University of Maryland, BaltimoreYuanyuan Liang - University of Maryland, BaltimoreDavid A. Rasko - University of Maryland, BaltimoreAnthony D. Harris - University of Maryland, Baltimore
- Resource Type
- Journal article
- Publication Details
- Infection control and hospital epidemiology, Vol.44(4), pp.578-588
- DOI
- 10.1017/ice.2022.159
- PMID
- 36177884
- NLM abbreviation
- Infect Control Hosp Epidemiol
- ISSN
- 0899-823X
- eISSN
- 1559-6834
- Publisher
- Cambridge Univ Press
- Number of pages
- 11
- Grant note
- U19AI110820; R01AI121146; 3R01AI221146-04S1 / National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID)
- Language
- English
- Date published
- 04/01/2023
- Academic Unit
- Epidemiology
- Record Identifier
- 9985123939602771
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