Journal article
Molecular determinants for lymph node metastasis in clinically early-stage endometrial cancer
Oncology Letters, Vol.11(1), pp.323-329
01/2016
DOI: 10.3892/ol.2015.3883
PMCID: PMC4726972
PMID: 26870211
Abstract
Patients with occult lymph node metastasis in endometrioid-type endometrial cancer (EC) are prone to the development of recurrences and have worse outcomes compared with patients without lymph node metastasis. In the current study, the aim was to identify molecular parameters associated with lymph node metastasis in EC clinically early-stage disease. A univariate analysis of differentially expressed genes, proteins and clinicopathological parameters (including myometrial invasion and tumor grade) was performed, comparing EC patients with and without lymph node metastasis (n=262 patients from The Cancer Genome Atlas). Significant parameters were introduced in a multivariate model and a gene expression pathway analysis. Lymph node metastasis was associated with expression of 268 unique genes (P<0.001), 19 unique proteins (P<0.05), tumor grade and myometrial invasion in univariate analysis. Multivariate analysis demonstrated 10 genes independently associated with lymph node metastasis and 4 independently associated proteins. Myometrial invasion was the only independent clinicopathological parameter associated with lymph node status. The enrichment pathway analysis demonstrated that expression of epidermal growth factor receptor, Bcl2 antagonist of cell death and phosphatase and tensin homolog pathways were significantly involved in lymph node metastasis (P≤0.001). A gene expression signature to predict lymph node status in EC was created for future validation. Few studies have focused on the association between EC's molecular characteristics and nodal metastasis. Defining molecular risk factors for EC lymphatic nodal metastasis may help to individualize treatment and improve patient outcomes.
Details
- Title: Subtitle
- Molecular determinants for lymph node metastasis in clinically early-stage endometrial cancer
- Creators
- NADIM BOU ZGHEIB - Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Iowa Hospital and Clinics, Iowa, IA 52242, USADOUGLAS C MARCHION - Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Iowa Hospital and Clinics, Iowa, IA 52242, USASTEPHEN H BUSH - Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Iowa Hospital and Clinics, Iowa, IA 52242, USAPATRICIA L JUDSON - Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Iowa Hospital and Clinics, Iowa, IA 52242, USAROBERT M WENHAM - Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Iowa Hospital and Clinics, Iowa, IA 52242, USASACHIN M APTE - Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Iowa Hospital and Clinics, Iowa, IA 52242, USAJOHNATHAN M LANCASTER - Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Iowa Hospital and Clinics, Iowa, IA 52242, USAJESUS GONZALEZ-BOSQUET - Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Iowa Hospital and Clinics, Iowa, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Oncology Letters, Vol.11(1), pp.323-329
- Publisher
- D.A. Spandidos
- DOI
- 10.3892/ol.2015.3883
- PMID
- 26870211
- PMCID
- PMC4726972
- ISSN
- 1792-1074
- eISSN
- 1792-1082
- Language
- English
- Date published
- 01/2016
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9983931820302771
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