Molecular evidence that granuloma T lymphocytes in murine schistosomiasis mansoni express an authentic substance P (NK-1) receptor

George A Cook; David Elliott; Ahmed Metwali; Arthur M Blum; Matyas Sandor; Richard Lynch; Joel V Weinstock

doi:10.4049/jimmunol.152.4.1830

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Molecular evidence that granuloma T lymphocytes in murine schistosomiasis mansoni express an authentic substance P (NK-1) receptor

Journal article

Peer reviewed

Molecular evidence that granuloma T lymphocytes in murine schistosomiasis mansoni express an authentic substance P (NK-1) receptor

George A Cook, David Elliott, Ahmed Metwali, Arthur M Blum, Matyas Sandor, Richard Lynch and Joel V Weinstock

The Journal of immunology (1950), Vol.152(4), pp.1830-1835

1994

DOI: 10.4049/jimmunol.152.4.1830

PMID: 8120392

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Abstract

Murine Schistosomiasis mansoni is a parasitic disease in which granulomas develop around the schistosome ova that lodge in the liver and intestines of the host. The granuloma eosinophils make substance P (SP), a cytokine with immunoregulatory properties. Within the granuloma SP can modulate IFN-gamma production through interaction with a substance P-like receptor. SP belongs to a family of hormones called tachykinins. Three mammalian tachykinins are SP, neurokinin A (substance K), and neurokinin B (neuromedin K). In humans and rats, there are at least three distinct tachykinin receptors designated NK-1, NK-2, and NK-3. The NK-1 receptor binds only SP with high affinity. Using reverse transcription-PCR, cDNA cloning, and sequence analysis, we showed that granulomas isolated from the liver of infected mice express an authentic SP (NK-1) receptor but have no detectable neurokinin A (NK-2) and neurokinin B (NK-3) receptor mRNA, as determined by PCR. CD4+ granuloma T lymphocytes, purified by FACS, express NK-1 receptor mRNA. Normal liver devoid of granulomas exhibited none of the three tachykinin receptor subclasses.

Biological and medical sciences

Cell receptors

Cell structures and functions

Fundamental and applied biological sciences. Psychology

Molecular and cellular biology

Neuropeptide receptors

Details

Title: Subtitle: Molecular evidence that granuloma T lymphocytes in murine schistosomiasis mansoni express an authentic substance P (NK-1) receptor
Creators: George A Cook - University of Wisconsin–Madison
David Elliott - Univ. Wisconsin, dep. animal health biomedical sci., Madison WI 53706, United States
Ahmed Metwali - Univ. Wisconsin, dep. animal health biomedical sci., Madison WI 53706, United States
Arthur M Blum - Univ. Wisconsin, dep. animal health biomedical sci., Madison WI 53706, United States
Matyas Sandor - Univ. Wisconsin, dep. animal health biomedical sci., Madison WI 53706, United States
Richard Lynch - Univ. Wisconsin, dep. animal health biomedical sci., Madison WI 53706, United States
Joel V Weinstock - Univ. Wisconsin, dep. animal health biomedical sci., Madison WI 53706, United States
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.152(4), pp.1830-1835
DOI: 10.4049/jimmunol.152.4.1830
PMID: 8120392
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: American Association of Immunologists
Language: English
Date published: 1994
Academic Unit: Gastroenterology and Hepatology; Internal Medicine
Record Identifier: 9984359816802771

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