Journal article
Molecular heterogeneity in the choroid plexus epithelium: the 22-member γ-protocadherin family is differentially expressed, apically localized, and implicated in CSF regulation
Journal of neurochemistry, Vol.120(6), pp.913-927
03/2012
DOI: 10.1111/j.1471-4159.2011.07587.x
PMCID: PMC3296866
PMID: 22092001
Abstract
The choroid plexus (CP) epithelium develops from the ependyma that lines the ventricular system, and plays a critical role in the development and function of the brain. In addition to being the primary site of CSF production, the CP maintains the blood-CSF barrier via apical tight junctions between epithelial cells. Here we show that the 22-member γ-Protocadherin (γ-Pcdh) family of cell adhesion molecules, which we have implicated previously in synaptogenesis and neuronal survival, is highly expressed by both CP epithelial and ependymal cells, in which γ-Pcdh protein localization is, surprisingly, tightly restricted to the apical membrane. Multi-label immunostaining demonstrates that γ-Pcdhs are excluded from tight junctions, basolateral adherens junctions, and apical cilia tufts. RT-PCR analysis indicates that, as a whole, the CP expresses most members of the
Pcdh-γ
gene family. Immunostaining using novel monoclonal antibodies specific for single γ-Pcdh proteins shows that individual epithelial cells differ in their apically-localized γ-Pcdh repertoire. Restricted mutation of the
Pcdh-γ
locus in the choroid plexus and ependyma leads to significant reductions in ventricular volume, without obvious disruptions of epithelial apical-basal polarity. Together, these results suggest an unsuspected role for the γ-Pcdhs in CSF production and demonstrate a surprising molecular heterogeneity in the CP epithelium.
Details
- Title: Subtitle
- Molecular heterogeneity in the choroid plexus epithelium: the 22-member γ-protocadherin family is differentially expressed, apically localized, and implicated in CSF regulation
- Creators
- Mark A Lobas - Department of Biology, The University of Iowa, Iowa City, IA 52242, USALindsey Helsper - Department of Biology, The University of Iowa, Iowa City, IA 52242, USAClaire G Vernon - Department of Biology, The University of Iowa, Iowa City, IA 52242, USADietmar Schreiner - Department of Biology, The University of Iowa, Iowa City, IA 52242, USAYong Zhang - Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USAMichael J Holtzman - Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USADaniel R Thedens - Department of Radiology, Carver College of Medicine, The University of Iowa, Iowa City, IA 52242, USAJoshua A Weiner - Department of Biology, The University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Journal of neurochemistry, Vol.120(6), pp.913-927
- DOI
- 10.1111/j.1471-4159.2011.07587.x
- PMID
- 22092001
- PMCID
- PMC3296866
- ISSN
- 0022-3042
- eISSN
- 1471-4159
- Grant note
- R01 NS055272-01A2 || NS / National Institute of Neurological Disorders and Stroke : NINDS
- Language
- English
- Date published
- 03/2012
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Liberal Arts and Science Admin; Electrical and Computer Engineering; Psychiatry; Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984065482102771
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