Molecular techniques drive cutting edge advancements in management of cutaneous T cell lymphoma

Mitchell N. Lefebvre; Nicholas Borcherding; Ryan J. Reis; Eric Mou; Vincent Liu; Ali Jabbari

doi:10.3389/fimmu.2023.1228563

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Molecular techniques drive cutting edge advancements in management of cutaneous T cell lymphoma

Journal article

Open access

Peer reviewed

Molecular techniques drive cutting edge advancements in management of cutaneous T cell lymphoma

Mitchell N. Lefebvre, Nicholas Borcherding, Ryan J. Reis, Eric Mou, Vincent Liu and Ali Jabbari

Frontiers in immunology, Vol.14, 1228563

08/01/2023

DOI: 10.3389/fimmu.2023.1228563

PMCID: PMC10465366

PMID: 37654486

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Abstract

Cutaneous 5T cell lymphoma (CTCL), characterized by malignant T cells infiltrating the skin with potential for dissemination, remains a challenging disease to diagnose and treat due to disease heterogeneity, treatment resistance, and lack of effective and standardized diagnostic and prognostic clinical tools. Currently, diagnosis of CTCL practically relies on clinical presentation, histopathology, and immunohistochemistry. These methods are collectively fraught with limitations in sensitivity and specificity. Fortunately, recent advances in flow cytometry, polymerase chain reaction, high throughput sequencing, and other molecular techniques have shown promise in improving diagnosis and treatment of CTCL. Examples of these advances include T cell receptor clonotyping via sequencing to detect CTCL earlier in the disease course and single-cell RNA sequencing to identify gene expression patterns that commonly drive CTCL pathogenesis. Experience with these techniques has afforded novel insights which may translate into enhanced diagnostic and therapeutic approaches for CTCL.

Flow Cytometry

cutaneous T cell lymphoma

high throughput sequencing

RNA sequencing

TCR - T cell receptor

Details

Title: Subtitle: Molecular techniques drive cutting edge advancements in management of cutaneous T cell lymphoma
Creators: Mitchell N. Lefebvre - University of Iowa
Nicholas Borcherding - Washington University in St. Louis
Ryan J. Reis - University of Iowa
Eric Mou - University of Iowa
Vincent Liu - University of Iowa
Ali Jabbari - University of Iowa
Resource Type: Journal article
Publication Details: Frontiers in immunology, Vol.14, 1228563
DOI: 10.3389/fimmu.2023.1228563
PMID: 37654486
PMCID: PMC10465366
NLM abbreviation: Front Immunol
eISSN: 1664-3224
Publisher: Frontiers Media S.A
Grant note: DOI: 10.13039/100000069, name: National Institute of Arthritis and Musculoskeletal and Skin Diseases; DOI: 10.13039/100000738, name: U.S. Department of Veterans Affairs; DOI: 10.13039/100000002, name: National Institutes of Health
Language: English
Date published: 08/01/2023
Academic Unit: Dermatology; Hematology, Oncology, and Blood & Marrow Transplantation; Pathology; Internal Medicine
Record Identifier: 9984456077402771

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