Journal article
Molecularly cleavable bioinks facilitate high-performance digital light processing-based bioprinting of functional volumetric soft tissues
Nature communications, Vol.13(1), pp.3317-3317
06/09/2022
DOI: 10.1038/s41467-022-31002-2
PMCID: PMC9184597
PMID: 35680907
Abstract
Digital light processing bioprinting favors biofabrication of tissues with improved structural complexity. However, soft-tissue fabrication with this method remains a challenge to balance the physical performances of the bioinks for high-fidelity bioprinting and suitable microenvironments for the encapsulated cells to thrive. Here, we propose a molecular cleavage approach, where hyaluronic acid methacrylate (HAMA) is mixed with gelatin methacryloyl to achieve high-performance bioprinting, followed by selectively enzymatic digestion of HAMA, resulting in tissue-matching mechanical properties without losing the structural complexity and fidelity. Our method allows cellular morphological and functional improvements across multiple bioprinted tissue types featuring a wide range of mechanical stiffness, from the muscles to the brain, the softest organ of the human body. This platform endows us to biofabricate mechanically precisely tunable constructs to meet the biological function requirements of target tissues, potentially paving the way for broad applications in tissue and tissue model engineering.
Details
- Title: Subtitle
- Molecularly cleavable bioinks facilitate high-performance digital light processing-based bioprinting of functional volumetric soft tissues
- Creators
- Mian Wang - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USAWanlu Li - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USAJin Hao - Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, USAArthur Gonzales III - University of the Philippines Diliman, Quezon City, Metro Manila, PhilippinesZhibo Zhao - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USARegina Sanchez Flores - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USAXiao Kuang - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USAXuan Mu - Harvard Medical SchoolTerry Ching - Department of Biomedical Engineering, National University of Singapore, Singapore, SingaporeGuosheng Tang - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USAZeyu Luo - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USACarlos Ezio Garciamendez-Mijares - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USAJugal Kishore Sahoo - Department of Biomedical Engineering, Tufts University, Medford, MA, USAMichael F Wells - Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, USAGengle Niu - Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USAPrajwal Agrawal - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USAAlfredo Quiñones-Hinojosa - Departments of Neurosurgery, Oncology, Neuroscience, Mayo Clinic, Jacksonville, FL, USAKevin Eggan - Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, USAYu Zhang - Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA. yszhang@research.bwh.harvard.edu
- Resource Type
- Journal article
- Publication Details
- Nature communications, Vol.13(1), pp.3317-3317
- DOI
- 10.1038/s41467-022-31002-2
- PMID
- 35680907
- PMCID
- PMC9184597
- NLM abbreviation
- Nat Commun
- ISSN
- 2041-1723
- eISSN
- 2041-1723
- Grant note
- DOI: 10.13039/100000002, name: U.S. Department of Health & Human Services | National Institutes of Health, award: R21EB026175, R21EB025270, R00CA201603, R01EB028143; name: U.S. Department of Health & Human Services | National Institutes of Health; name: U.S. Department of Health & Human Services | National Institutes of Health; name: U.S. Department of Health & Human Services | National Institutes of Health; DOI: 10.13039/100000001, name: National Science Foundation, award: CBET-EBMS-123859; DOI: 10.13039/100008552, name: BWH | Brigham Research Institute, award: NA
- Language
- English
- Date published
- 06/09/2022
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9984304558902771
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