Monoclonal anti-DNA IgMκ in neuropathy binds to myelin and to a conformational epitope formed by phosphatidic acid and gangliosides

Lorenza Freddo; Arthur P Hays; Katherine G Nickerson; Linda Spatz; Scott McGinnis; Rebecca Lieberson; Christian A Vedeler; Michael E Shy; Lucila Autilio-Gambetti; Frances C Grauss; Frank Petito; Leonard Chess; Norman Latov

doi:10.4049/jimmunol.137.12.3821

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Monoclonal anti-DNA IgMκ in neuropathy binds to myelin and to a conformational epitope formed by phosphatidic acid and gangliosides

Journal article

Peer reviewed

Monoclonal anti-DNA IgMκ in neuropathy binds to myelin and to a conformational epitope formed by phosphatidic acid and gangliosides

Lorenza Freddo, Arthur P Hays, Katherine G Nickerson, Linda Spatz, Scott McGinnis, Rebecca Lieberson, Christian A Vedeler, Michael E Shy, Lucila Autilio-Gambetti, Frances C Grauss, …

The Journal of immunology (1950), Vol.137(12), pp.3821-3825

1986

DOI: 10.4049/jimmunol.137.12.3821

PMID: 2431044

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Abstract

Anti-DNA antibodies that cross-react with phosphorylated epitopes of other cellular constituents may be involved in the pathogenesis of autoimmune disease. An IgM monoclonal antibody from a patient with chronic lymphocytic leukemia (CLL) and neuropathy bound to denatured DNA and immunostained myelin in peripheral nerve and spinal cord. The monoclonal IgM bound to ELISA microwells coated with a mixture of phosphatidic acid and gangliosides at serum dilutions of up to 1/100,000, but binding to phosphatidic acid alone was observed at dilutions of less than 1/100 only, and there was no binding to gangliosides alone. Incubation with micelles containing phosphatidic acid and gangliosides selectively absorbed the monoclonal IgM and inhibited its binding to denatured DNA and to myelin. These observations suggest that autoantibodies may bind to conformational epitopes formed by two separate molecules, and that autoantibodies that cross-react with phosphorylated epitopes in DNA and neural tissue could be involved in autoimmune neurologic diseases.

Neurology

Cranial nerves. Spinal roots. Peripheral nerves. Autonomic nervous system. Gustation. Olfaction

Biological and medical sciences

Medical sciences

Nervous system (semeiology, syndromes)

Details

Title: Subtitle: Monoclonal anti-DNA IgMκ in neuropathy binds to myelin and to a conformational epitope formed by phosphatidic acid and gangliosides
Creators: Lorenza Freddo - Columbia univ., dep. neurology, New York NY 10032, United States
Arthur P Hays - Columbia univ., dep. neurology, New York NY 10032, United States
Katherine G Nickerson - Columbia univ., dep. neurology, New York NY 10032, United States
Linda Spatz - Columbia univ., dep. neurology, New York NY 10032, United States
Scott McGinnis - Columbia univ., dep. neurology, New York NY 10032, United States
Rebecca Lieberson - Columbia univ., dep. neurology, New York NY 10032, United States
Christian A Vedeler - Columbia univ., dep. neurology, New York NY 10032, United States
Michael E Shy - Columbia univ., dep. neurology, New York NY 10032, United States
Lucila Autilio-Gambetti - Columbia univ., dep. neurology, New York NY 10032, United States
Frances C Grauss - Columbia univ., dep. neurology, New York NY 10032, United States
Frank Petito - Columbia univ., dep. neurology, New York NY 10032, United States
Leonard Chess - Columbia univ., dep. neurology, New York NY 10032, United States
Norman Latov - Columbia univ., dep. neurology, New York NY 10032, United States
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.137(12), pp.3821-3825
Publisher: American Association of Immunologists; Bethesda, MD
DOI: 10.4049/jimmunol.137.12.3821
PMID: 2431044
ISSN: 0022-1767
eISSN: 1550-6606
Language: English
Date published: 1986
Academic Unit: Neurology; Molecular Physiology and Biophysics; Stead Family Department of Pediatrics; Iowa Neuroscience Institute
Record Identifier: 9984020778502771

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