Journal article
Monoclonal antibodies against three major glycoproteins of varicella-zoster virus
Infection and immunity, Vol.40(1), pp.381-388
04/1983
DOI: 10.1128/iai.40.1.381-388.1983
PMID: 6299963
Abstract
Varicella-zoster virus (VZV) codes for three prominent glycoproteins--gp62, gp98, and gp118--in infected cell cultures. To characterize individually these known immunogens, we first inoculated BALB/c mice with crude VZV extracts, produced hybridoma cultures by Köhler-Milstein cell-fusion technology, and screened culture supernatants by indirect immunofluorescence for reactivity directed against unfixed VZV-infected cells (FAMA assay). Supernatants from five independently derived and subcloned hybridomas with a high VZV-FAMA titer but no reactivity against either uninfected or herpes simplex virus-infected cells were further analyzed by immunoprecipitation of [3H]fucose-labeled and detergent-solubilized VZV antigen preparations. Fractionation of the precipitates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis demonstrated that four monoclonal antibodies reacted with both gp62 and gp98, and one precipitated only gp118. The profiles were unchanged whether performed under reducing or nonreducing conditions. When assayed for neutralizing activity, the secretory product of the single anti-gp118 hybridoma, but not the supernatants from the four anti-gp62/gp98 clones, inhibited VZV plaque formation by greater than 80%. Thus, at least one of the glycosylated antigens detected by the FAMA assay is a determinant which elicits neutralizing activity.
Details
- Title: Subtitle
- Monoclonal antibodies against three major glycoproteins of varicella-zoster virus
- Creators
- C GroseD P EdwardsW E FriedrichsK A WeigleW L McGuire
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.40(1), pp.381-388
- DOI
- 10.1128/iai.40.1.381-388.1983
- PMID
- 6299963
- NLM abbreviation
- Infect Immun
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Language
- English
- Date published
- 04/1983
- Academic Unit
- Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093463802771
Metrics
12 Record Views