Journal article
Monoclonal antibodies to dog heart sarcoplasmic reticulum as markers of endoplasmic reticulum
Journal of molecular and cellular cardiology, Vol.21(1), pp.55-58
1989
DOI: 10.1016/0022-2828(89)90837-7
PMID: 2543829
Abstract
A monoclonal antibody (mAb 4B4) was raised against purified sarcoplasmic reticulum vesicles from canine myocardium, and shown to inhibit Ca
2+ uptake by microsomes isolated from cardiac, skeletal, and smooth muscle. The amount of mAb 4B4 needed to inhibit the Ca
2+ uptake 50% at a given membrane concentration correlated with the amount of Ca
2+ pump protein in the microsomal preparation. This is consistent with the observation the mAb 4B4 binds specifically to the sarcoplasmic/endoplasmic reticulum Ca
2+ pump (M
r 100 kDa), but has no effect on the T-tubule Mg
2+-ATPase. Changes in the binding of mAb 4B4 to crude microsomes isolated from dog heart after various durations of global ischemia showed that the decrease in microsomal Ca
2+ transport during the first 15 min of ischemia correlated with a loss of active Ca
2+ pump molecules. The monoclonal antibody mAb 4B4 may therefore serve as a specific marker for the sarcoplasmic/endoplasmic reticulum Ca
2+ pump system in various cells, and can provide quantitative information about the loss of active Ca
2+ pump proteins under pathological conditions.
Details
- Title: Subtitle
- Monoclonal antibodies to dog heart sarcoplasmic reticulum as markers of endoplasmic reticulum
- Creators
- D.O Levitsky - Institute of Experimental Cardiology, Cardiology Research Center of the USSR, Academy of Medical Sciences, 3rd Cherepkovskaya Street 15A, Moscow 121552, USSRD.S Benevolensky - Institute of Experimental Cardiology, Cardiology Research Center of the USSR, Academy of Medical Sciences, 3rd Cherepkovskaya Street 15A, Moscow 121552, USSRN Ikemoto - Department of Muscle Research, Boston Biomedical Research Institute, Boston, MA, USAS.I Syrbu - Institute of Experimental Cardiology, Cardiology Research Center of the USSR, Academy of Medical Sciences, 3rd Cherepkovskaya Street 15A, Moscow 121552, USSRJ Watras - Department of Medicine, University of Connecticut Health Center, Farmington, CT, USA
- Resource Type
- Journal article
- Publication Details
- Journal of molecular and cellular cardiology, Vol.21(1), pp.55-58
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/0022-2828(89)90837-7
- PMID
- 2543829
- ISSN
- 0022-2828
- eISSN
- 1095-8584
- Language
- English
- Date published
- 1989
- Academic Unit
- Pathology
- Record Identifier
- 9984047653402771
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