Monocyte derived macrophages from CF pigs exhibit increased inflammatory responses at birth

Lily Paemka; Brian N McCullagh; Mahmoud H Abou Alaiwa; David A Stoltz; Qian Dong; Christoph O Randak; Robert D Gray; Paul B McCray

doi:10.1016/j.jcf.2017.03.007

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Monocyte derived macrophages from CF pigs exhibit increased inflammatory responses at birth

Journal article

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Peer reviewed

Monocyte derived macrophages from CF pigs exhibit increased inflammatory responses at birth

Lily Paemka, Brian N McCullagh, Mahmoud H Abou Alaiwa, David A Stoltz, Qian Dong, Christoph O Randak, Robert D Gray and Paul B McCray

Journal of cystic fibrosis, Vol.16(4), pp.471-474

07/2017

DOI: 10.1016/j.jcf.2017.03.007

PMCID: PMC5515361

PMID: 28377087

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Abstract

We sought to address whether CF macrophages have a primary functional defect as a consequence of CFTR loss and thus contribute to the onset of infection and inflammation observed in CF lung disease. Monocyte derived macrophages (MDMs) were prepared from newborn CF and non-CF pigs. CFTR mRNA expression was quantified by rtPCR and anion channel function was determined using whole cell patch clamp analysis. IL8 and TNFα release from MDMs in response to lipopolysaccharide stimulation was measured by ELISA. CFTR was expressed in MDMs by Q-rtPCR at a lower level than in epithelial cells. MDMs exhibited functional CFTR current at the cell membrane and this current was absent in CF MDMs. CF MDMs demonstrated an exaggerated response to lipopolysaccharide stimulation. In the absence of CFTR function, macrophages from newborn CF pigs exhibit an increased inflammatory response to a lipopolysaccharide challenge. This may contribute to the onset and progression of CF lung disease.

Lipopolysaccharide

Inflammation

Monocyte

CFTR

Macrophage

Details

Title: Subtitle: Monocyte derived macrophages from CF pigs exhibit increased inflammatory responses at birth
Creators: Lily Paemka - Department of Pediatrics, Pappajohn Biomedical Institute, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
Brian N McCullagh - Department of Pediatrics, Pappajohn Biomedical Institute, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
Mahmoud H Abou Alaiwa - Department of Internal Medicine, Pappajohn Biomedical Institute, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
David A Stoltz - Department of Internal Medicine, Pappajohn Biomedical Institute, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
Qian Dong - Department of Pediatrics, Pappajohn Biomedical Institute, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
Christoph O Randak - Department of Pediatrics, Pappajohn Biomedical Institute, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
Robert D Gray - Department of Pediatrics, Pappajohn Biomedical Institute, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
Paul B McCray - Department of Pediatrics, Pappajohn Biomedical Institute, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
Resource Type: Journal article
Publication Details: Journal of cystic fibrosis, Vol.16(4), pp.471-474
DOI: 10.1016/j.jcf.2017.03.007
PMID: 28377087
PMCID: PMC5515361
NLM abbreviation: J Cyst Fibros
ISSN: 1569-1993
eISSN: 1873-5010
Publisher: Elsevier B.V
Language: English
Date published: 07/2017
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Surgery; Internal Medicine
Record Identifier: 9984025361502771

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