Journal article
Monocyte responses to Candida albicans are enhanced by antibody in cooperation with antibody-independent pathogen recognition
FEMS immunology and medical microbiology, Vol.51(1), pp.70-83
10/01/2007
DOI: 10.1111/j.1574-695X.2007.00278.x
PMID: 17610517
Abstract
Abstract
Although most individuals are colonized with Candida albicans, only patients with insufficient or nonfunctional phagocytes develop life-threatening C. albicans disease. Because recognition of bacterial pathogens through phagocyte receptors for IgG (FcγR) is known to augment phagocyte responses, we postulated that antibody opsonization would enhance monocyte damage to C. albicans and subsequent tumor necrosis factor-α (TNF-α) production. After exposure to the human monocytic cell line THP-1, opsonized yeast showed an 89% decrease in metabolic activity, compared with 40% for unopsonized yeast (P<0.05). Culture supernatants contained 1316 pg mL−1 of TNF-α after monocytes were exposed to opsonized yeast vs. 341 pg mL−1 for unopsonized yeast (P=0.003). Similar results were obtained using peripheral blood mononuclear cells. Antibody opsonization of C. albicans germ tubes enhanced TNF-α production but did not affect organism damage. Antibody-dependent and antibody-independent factors were found to act synergistically to increase TNF-α production. ERK activation was important for both antibody-dependent and antibody-independent stimulation of TNF-α production, but not for monocyte-mediated organism damage. These data suggest that FcγR cooperates positively with antibody-independent recognition mechanisms in what may be a novel link between innate and adaptive immunity to C. albicans.
Details
- Title: Subtitle
- Monocyte responses to Candida albicans are enhanced by antibody in cooperation with antibody-independent pathogen recognition
- Creators
- Melanie Wellington - Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, NY, USAKristy Dolan - Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, NY, USAConstantine G Haidaris - Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA
- Resource Type
- Journal article
- Publication Details
- FEMS immunology and medical microbiology, Vol.51(1), pp.70-83
- Publisher
- Blackwell Publishing Ltd
- DOI
- 10.1111/j.1574-695X.2007.00278.x
- PMID
- 17610517
- ISSN
- 0928-8244
- eISSN
- 1574-695X
- Language
- English
- Date published
- 10/01/2007
- Academic Unit
- Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093339402771
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