Monocyte suppressor factor is plasminogen activator inhibitor inhibition of membrane bound but not soluble IL-1

Nancy E Goeken; Tina S Staggs; Zuhair K Ballas

doi:10.4049/jimmunol.143.2.603

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Monocyte suppressor factor is plasminogen activator inhibitor inhibition of membrane bound but not soluble IL-1

Journal article

Peer reviewed

Monocyte suppressor factor is plasminogen activator inhibitor inhibition of membrane bound but not soluble IL-1

Nancy E Goeken, Tina S Staggs and Zuhair K Ballas

The Journal of immunology (1950), Vol.143(2), pp.603-608

07/15/1989

DOI: 10.4049/jimmunol.143.2.603

PMID: 2525589

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Abstract

Human monocytes cultured in monolayer for 6 days were found to secrete a factor that suppressed the T cell proliferative response to soluble Ag and to alloantigens. The elaboration of this monocyte suppressor factor (MSF) was not inhibited by indomethacin. It has an apparent Mr of 50 to 60 kDa. It does not inhibit soluble IL-1 in the murine thymocyte costimulator assay but does inhibit the activity of membrane bound IL-1, which we observed to be almost exclusively IL-1 alpha. MSF contains elevated amounts of plasminogen activator inhibitor (PAI) when measured either as bioactivity or in an ELISA. Its immunosuppressive properties are inhibited by anti-PAI antibody. Furthermore, the eluate but not the effluent of an anti-PAI immunoabsorbent column contains all of the immunosuppressive activity. Based on these data we suggest that MSF is, in fact, PAI and postulate that the mechanism of action is inhibition of the plasmin cascade, thereby preventing the release of membrane bound IL-1. This suggests that monocytes possess an autoregulatory circuit that may have implications for the kinetics of the inflammatory response.

Binding, Competitive

Immune Sera - pharmacology

Interleukin-1 - metabolism

Gels

Immunosorbents

Humans

Immunosuppressive Agents - physiology

Cells, Cultured

Glycoproteins - antagonists & inhibitors

Immunosuppressive Agents - antagonists & inhibitors

Solubility

Monocytes - metabolism

Monocytes - immunology

Plasminogen Inactivators

Glycoproteins - physiology

Indomethacin - pharmacology

Lymphocyte Culture Test, Mixed

Glycoproteins - biosynthesis

Proteins

Immunosuppressive Agents - biosynthesis

Chemical Fractionation

Cell Membrane - metabolism

Interleukin-1 - antagonists & inhibitors

Details

Title: Subtitle: Monocyte suppressor factor is plasminogen activator inhibitor inhibition of membrane bound but not soluble IL-1
Creators: Nancy E Goeken - Department of Internal Medicine, Veterans Administration Medical Center, Iowa City 52240
Tina S Staggs
Zuhair K Ballas
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.143(2), pp.603-608
DOI: 10.4049/jimmunol.143.2.603
PMID: 2525589
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Grant note: AI 26460 / NIAID NIH HHS R01 DK33217 / NIDDK NIH HHS
Language: English
Date published: 07/15/1989
Academic Unit: Immunology; Internal Medicine
Record Identifier: 9984094722102771

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