Journal article
Monomeric C-reactive protein and inflammation in age-related macular degeneration
The Journal of pathology, Vol.240(2), pp.173-183
10/2016
DOI: 10.1002/path.4766
PMCID: PMC5527328
PMID: 27376713
Abstract
Age-related macular degeneration (AMD) is a devastating disease characterized by central vision loss in elderly individuals. Previous studies have suggested a link between elevated levels of total C-reactive protein (CRP) in the choroid, CFH genotype, and AMD status; however, the structural form of CRP present in the choroid, its relationship to CFH genotype, and its functional consequences have not been assessed. In this report, we studied genotyped human donor eyes (n = 60) and found that eyes homozygous for the high-risk CFH (Y402H) allele had elevated monomeric CRP (mCRP) within the choriocapillaris and Bruch's membrane, compared to those with the low-risk genotype. Treatment of choroidal endothelial cells in vitro with mCRP increased migration rate and monolayer permeability compared to treatment with pentameric CRP (pCRP) or medium alone. Organ cultures treated with mCRP exhibited dramatically altered expression of inflammatory genes as assessed by RNA sequencing, including ICAM-1 and CA4, both of which were confirmed at the protein level. Our data indicate that mCRP is the more abundant form of CRP in human choroid, and that mCRP levels are elevated in individuals with the high-risk CFH genotype. Moreover, pro-inflammatory mCRP significantly affects endothelial cell phenotypes in vitro and ex vivo, suggesting a role for mCRP in choroidal vascular dysfunction in AMD. Copyright © 2016 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Details
- Title: Subtitle
- Monomeric C-reactive protein and inflammation in age-related macular degeneration
- Creators
- Kathleen R Chirco - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, Iowa, USAS Scott Whitmore - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, Iowa, USAKai Wang - Department of Biostatistics, The University of Iowa, Iowa City, Iowa, USALawrence A Potempa - Roosevelt University College of Pharmacy, Schaumburg, Illinois, USAJennifer A Halder - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, Iowa, USAEdwin M Stone - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, Iowa, USABudd A Tucker - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, Iowa, USARobert F Mullins - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, Iowa, USA. Robert-Mullins@uiowa.edu
- Resource Type
- Journal article
- Publication Details
- The Journal of pathology, Vol.240(2), pp.173-183
- DOI
- 10.1002/path.4766
- PMID
- 27376713
- PMCID
- PMC5527328
- NLM abbreviation
- J Pathol
- ISSN
- 0022-3417
- eISSN
- 1096-9896
- Publisher
- England
- Grant note
- R01 EY024605 / NEI NIH HHS
- Language
- English
- Date published
- 10/2016
- Academic Unit
- Iowa Neuroscience Institute; Biostatistics; Ophthalmology and Visual Sciences
- Record Identifier
- 9983980065702771
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