Journal article
Morphologic and Histopathologic Changes in the Rabbit Cornea Produced by Femtosecond Laser-Assisted Multilayer Intrastromal Ablation
Investigative ophthalmology & visual science, Vol.50(5), pp.2147-2153
05/01/2009
DOI: 10.1167/iovs.08-2400
PMID: 19136715
Abstract
PURPOSE. To observe morphologic and histopathologic changes in the midperiphery of the rabbit cornea produced by femtosecond laser-assisted multilayer intrastromal ablation, determine whether this method may be used to correct myopia, and study how the cornea heals when the epithelium is not injured.
METHODS. The right eyes of 10 New Zealand White rabbits were used for the experiments. A 60-kHz femtosecond laser delivery system was used, and three lamellar layers of laser pulses were focused starting at a corneal depth of 180 mu m and ending at 90 mu m from the surface, with each successive layer placed 45 mu m anterior to the previous layer. In the interface of the applanation contact lens cone, a 6-mm diameter aluminum circle was placed at the center to block the laser, and ablation was limited to the midperiphery of the cornea. The laser settings were spot/line separation, 10 mu m; diameter, 8.5 mm; energy for ablating the stroma, 1.3 mu J. Topography examination was used to document changes in corneal power. Light microscopy, transmission electron microscopy (TEM), and confocal microscopy in vivo were applied to observe changes in the cornea.
RESULTS. There was significant change in mean corneal power between baseline and postoperative month 3 (n = 8; P = 0.0001), with a decrease from 46.82 D to 44.42 D. There was no haze formation or refractive regression throughout the follow-up. There were no corneal structural abnormalities under light microscopy. Activated keratocytes and necrotic debris were visible under confocal microscopy. Fibroblasts were observed, and no myofibroblasts appeared under TEM.
CONCLUSIONS. Multilayer intrastromal ablation by the femtosecond laser with intact epithelium in the midperiphery of the corneal stroma can flatten the cornea without causing haze formation or refractive regression. This procedure allows the cornea to heal differently than when traditional corneal refractive surgery is performed and the epithelium is damaged. (Invest Ophthalmol Vis Sci. 2009;50:2147-2153) DOI:10.1167/iovs.08-2400
Details
- Title: Subtitle
- Morphologic and Histopathologic Changes in the Rabbit Cornea Produced by Femtosecond Laser-Assisted Multilayer Intrastromal Ablation
- Creators
- Zhen-Yong Zhang - Fudan UniversityRen-Yuan Chu - Eye & ENT Hospital of Fudan UniversityXing-Tao Zhou - Fudan UniversityJin-Hui Dai - Fudan UniversityXing-Huai Sun - Fudan UniversityMatthew R. Hoffman - University of Wisconsin School of Medicine and Public HealthXing-Ru Zhang - Shanghai Traditional Chinese Medicine Hospital
- Resource Type
- Journal article
- Publication Details
- Investigative ophthalmology & visual science, Vol.50(5), pp.2147-2153
- Publisher
- Assoc Research Vision Ophthalmology Inc
- DOI
- 10.1167/iovs.08-2400
- PMID
- 19136715
- ISSN
- 0146-0404
- eISSN
- 1552-5783
- Number of pages
- 7
- Grant note
- NCET-07-0212 / Plan for the New Century Excellent Teacher of the Educational Ministry of People's Republic of China 30530770 / Chinese Natural Science Fund Committee; National Natural Science Foundation of China (NSFC) Grant 05dz22325 / Shanghai Science and Technology Committee; Shanghai Science & Technology Committee EYF158017 / Fudan University Postgraduate Creative Fund Program
- Language
- English
- Date published
- 05/01/2009
- Academic Unit
- Otolaryngology
- Record Identifier
- 9984366279802771
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