Journal article
Morphological and structural characterization of a polysaccharide from Gynostemma pentaphyllum Makino and its anti-exercise fatigue activity
Carbohydrate polymers, Vol.74(4), pp.868-874
11/21/2008
DOI: 10.1016/j.carbpol.2008.05.010
Abstract
The crude polysaccharide was obtained from Gynostemma pentaphyllum Makino by water extraction followed by ethanol precipitation. The polysaccharide was successively purified by chromatography on DEAE-52 and SephadexG-150 column, and three polysaccharide fractions were obtained and termed GPP1-a, GPP2-b, and GPP3-a, respectively. The administration with GPP1-a markedly prolonged exhaustive exercise time of the mice. Structural features of GPP1-a were investigated by a combination of instrumental and chemical analyses, including atomic force microscope (AFM), scanning electron microscope (SEM), partial acid hydrolysis, periodate oxidation, Smith degradation, methylation analysis, gas chromatography-mass spectrometry (GC-MS) analysis and NMR spectroscopy. The results indicate that GPP1-a has a backbone of (1 -> 4)-linked alpha-D-Glucose residues. which occasionally branches at O-6. The branches are mainly composed of (1 -> 6)-linked alpha-D-Glucose, (1 -> 3)-linked beta-D-Galactose and (1 -> 6)-linked alpha-Galactose residues, and terminated with P-D-Galactose residues and P-L-Arabinose residues. (C) 2008 Elsevier Ltd. All rights reserved.
Details
- Title: Subtitle
- Morphological and structural characterization of a polysaccharide from Gynostemma pentaphyllum Makino and its anti-exercise fatigue activity
- Creators
- Ai-Ping Chi - Shaanxi Normal UniversityJin-Ping Chen - Shaanxi Normal UniversityZhe-Zhi Wang - Chang'an UniversityZheng-Yi Xiong - Shaanxi Normal UniversityQiu-Xia Li - Shaanxi Normal University
- Resource Type
- Journal article
- Publication Details
- Carbohydrate polymers, Vol.74(4), pp.868-874
- Publisher
- Elsevier
- DOI
- 10.1016/j.carbpol.2008.05.010
- ISSN
- 0144-8617
- eISSN
- 1879-1344
- Number of pages
- 7
- Grant note
- 2006BA106A12 / "11th Five-Year" Scientific and Technological Support Plan; National Key Technology R&D Program
- Language
- English
- Date published
- 11/21/2008
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984360043802771
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