Morphological integration and functional assessment of transplanted neural progenitor cells in healthy and acute ischemic rat eyes

Sinisa D Grozdanic; Allison M Ast; Tatjana Lazic; Young H Kwon; Randy H Kardon; Ioana M Sonea; Donald S Sakaguchi

doi:10.1016/j.exer.2005.08.020

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Morphological integration and functional assessment of transplanted neural progenitor cells in healthy and acute ischemic rat eyes

Journal article

Peer reviewed

Morphological integration and functional assessment of transplanted neural progenitor cells in healthy and acute ischemic rat eyes

Sinisa D Grozdanic, Allison M Ast, Tatjana Lazic, Young H Kwon, Randy H Kardon, Ioana M Sonea and Donald S Sakaguchi

Experimental Eye Research, Vol.82(4), pp.597-607

2006

DOI: 10.1016/j.exer.2005.08.020

PMID: 16213484

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Abstract

We have functionally and morphologically characterized the retina and optic nerve after neural progenitor cell transplants to healthy rat eyes and eyes damaged by acute elevation of intraocular pressure (IOP). Green fluorescent protein-expressing adult rat hippocampal progenitor cells (AHPCs) were transplanted by intravitreal injection into healthy eyes and eyes damaged with acute ocular hypertension. Pupil light reflexes (PLR) and electroretinograms (ERGs) were recorded preoperatively and postoperatively. Eyes were subsequently prepared for immunohistochemical analysis and confocal imaging. Transplanted AHPCs were found in 8 of 15 (53%) acute ischemic eyes 62 days after surgery and 5 of 10 (50%) healthy eyes 32 days after grafting. Analysis of PLR and ERG function in acute ischemic eyes revealed no statistically significant difference compared to controls after transplantation for all observed functional parameters. Transplant into healthy rat eyes revealed no PLR or ERG amplitude deficits between transplanted and non-transplanted (control) eyes. Morphological and immunohistochemical analysis revealed that transplanted AHPCs survived and differentiated in both normal and injured retinal environments. Morphological integration occurred primarily within the inner retinal layers of the acute ischemic eyes. AHPCs were found to express neuronal and glial markers following transplantation. Transplanted AHPCs have the ability to integrate and differentiate in ischemia damaged retinas. PLR and ERG analysis revealed no significant difference in functional outcome in transplant recipient eyes.

electroretinogram

pupillometry

stem cells

retinal ischemia

optic nerve

Details

Title: Subtitle: Morphological integration and functional assessment of transplanted neural progenitor cells in healthy and acute ischemic rat eyes
Creators: Sinisa D Grozdanic - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
Allison M Ast - Neuroscience Program and Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA
Tatjana Lazic - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
Young H Kwon - Department of Ophthalmology and Visual Sciences, University of Iowa Hospitals and Clinics, Iowa City, IA 52242, USA
Randy H Kardon - Department of Ophthalmology and Visual Sciences, University of Iowa Hospitals and Clinics, Iowa City, IA 52242, USA
Ioana M Sonea - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
Donald S Sakaguchi - Neuroscience Program and Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA
Resource Type: Journal article
Publication Details: Experimental Eye Research, Vol.82(4), pp.597-607
DOI: 10.1016/j.exer.2005.08.020
PMID: 16213484
NLM abbreviation: Exp Eye Res
ISSN: 0014-4835
eISSN: 1096-0007
Publisher: Elsevier Ltd
Language: English
Date published: 2006
Academic Unit: Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
Record Identifier: 9983980298502771

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