Journal article
Morphometric differences between central vs. surface acini in A/J mice using high-resolution micro-computed tomography
Journal of applied physiology (1985), Vol.121(1), pp.115-122
07/01/2016
DOI: 10.1152/japplphysiol.00317.2016
PMCID: PMC4967250
PMID: 27174924
Abstract
Through interior tomography, high-resolution microcomputed tomography (μCT) systems provide the ability to nondestructively assess the pulmonary acinus at micron and submicron resolutions. With the application of systematic uniform random sampling (SURS) principles applied to in situ fixed, intact, ex vivo lungs, we have sought to characterize morphometric differences in central vs. surface acini to better understand how well surface acini reflect global acinar geometry. Lungs from six mice (A/J strain, 15-20 wk of age) were perfusion fixed in situ and imaged using a multiresolution μCT system (Micro XCT 400, Zeiss). With the use of lower-resolution whole lung images, SURS methods were used for identification of central and surface foci for high-resolution imaging. Acinar morphometric metrics included diameters, lengths, and branching angles for each alveolar duct and total path lengths from entrance of the acinus to the terminal alveolar sacs. In addition, acinar volume, alveolar surface area, and surface area/volume ratios were assessed. A generation-based analysis demonstrated that central acini have significantly smaller branch diameters at each generation with no significant increase in branch lengths. In addition to larger-diameter alveolar ducts, surface acini had significantly increased numbers of branches and terminal alveolar sacs. The total path lengths from the acinar entrance to the terminal nodes were found to be higher in the case of surface acini. Volumes and surface areas of surface acini are greater than central acini, but there were no differences in surface/volume ratios. In conclusion, there are significant structural differences between surface and central acini in the A/J mouse.
Details
- Title: Subtitle
- Morphometric differences between central vs. surface acini in A/J mice using high-resolution micro-computed tomography
- Creators
- Abhilash S Kizhakke Puliyakote - Department of Radiology, University of Iowa, Iowa City, Iowa; Department of Biomedical Engineering, University of Iowa, Iowa City, IowaDragoş M Vasilescu - Department of Radiology, University of Iowa, Iowa City, IowaJohn D Newell Jr - Department of Radiology, University of Iowa, Iowa City, Iowa; Department of Biomedical Engineering, University of Iowa, Iowa City, IowaGe Wang - Department of Electrical and Computer Engineering, Virginia Polytechnic Institute and State University, Blacksburg, Virginia; Department of Biomedical Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VirginiaEwald R Weibel - University of Bern, Bern, SwitzerlandEric A Hoffman - Department of Radiology, University of Iowa, Iowa City, Iowa; Department of Biomedical Engineering, University of Iowa, Iowa City, Iowa; Department of Medicine, University of Iowa, Iowa City, Iowa; eric-hoffman@uiowa.edu
- Resource Type
- Journal article
- Publication Details
- Journal of applied physiology (1985), Vol.121(1), pp.115-122
- Publisher
- United States
- DOI
- 10.1152/japplphysiol.00317.2016
- PMID
- 27174924
- PMCID
- PMC4967250
- ISSN
- 8750-7587
- eISSN
- 1522-1601
- Grant note
- R01 HL112986 / NHLBI NIH HHS S10 OD018503 / NIH HHS
- Language
- English
- Date published
- 07/01/2016
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Internal Medicine
- Record Identifier
- 9984051576402771
Metrics
15 Record Views