Journal article
Motile Properties of Vimentin Intermediate Filament Networks in Living Cells
The Journal of cell biology, Vol.143(1), pp.147-157
10/05/1998
DOI: 10.1083/jcb.143.1.147
PMCID: PMC2132819
PMID: 9763427
Abstract
The motile properties of intermediate filament (IF) networks have been studied in living cells expressing vimentin tagged with green fluorescent protein (GFP-vimentin). In interphase and mitotic cells, GFP-vimentin is incorporated into the endogenous IF network, and accurately reports the behavior of IF. Time-lapse observations of interphase arrays of vimentin fibrils demonstrate that they are constantly changing their configurations in the absence of alterations in cell shape. Intersecting points of vimentin fibrils, or foci, frequently move towards or away from each other, indicating that the fibrils can lengthen or shorten. Fluorescence recovery after photobleaching shows that bleach zones across fibrils rapidly recover their fluorescence. During this recovery, bleached zones frequently move, indicating translocation of fibrils. Intriguingly, neighboring fibrils within a cell can exhibit different rates and directions of movement, and they often appear to extend or elongate into the peripheral regions of the cytoplasm. In these same regions, short filamentous structures are also seen actively translocating. All of these motile properties require energy, and the majority appear to be mediated by interactions of IF with microtubules and microfilaments.
Details
- Title: Subtitle
- Motile Properties of Vimentin Intermediate Filament Networks in Living Cells
- Creators
- Miri Yoon - Northwestern University Medical School, Department of Cell and Molecular Biology, Chicago, Illinois 60611Robert D Moir - Northwestern University Medical School, Department of Cell and Molecular Biology, Chicago, Illinois 60611Veena Prahlad - Northwestern University Medical School, Department of Cell and Molecular Biology, Chicago, Illinois 60611Robert D Goldman - Northwestern University Medical School, Department of Cell and Molecular Biology, Chicago, Illinois 60611
- Resource Type
- Journal article
- Publication Details
- The Journal of cell biology, Vol.143(1), pp.147-157
- Publisher
- The Rockefeller University Press
- DOI
- 10.1083/jcb.143.1.147
- PMID
- 9763427
- PMCID
- PMC2132819
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Language
- English
- Date published
- 10/05/1998
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984070836002771
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