Mouse epidermal stem cells proceed through the cell cycle

Martine Dunnwald; Sathivel Chinnathambi; Dana Alexandrunas; Jackie R Bickenbach

doi:10.1002/jcp.10311

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Mouse epidermal stem cells proceed through the cell cycle

Journal article

Peer reviewed

Mouse epidermal stem cells proceed through the cell cycle

Martine Dunnwald, Sathivel Chinnathambi, Dana Alexandrunas and Jackie R Bickenbach

Journal of cellular physiology, Vol.195(2), pp.194-201

05/2003

DOI: 10.1002/jcp.10311

PMID: 12652646

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Abstract

The epidermis is a continuously renewing tissue maintained by undifferentiated stem cells. For decades it has been assumed that epidermal stem cells (ESCs) were held in the G0 phase of the cell cycle and that they only entered the cell cycle when needed. Previously, we showed that ESCs retained nuclear label for long periods, indicating that these cells did not proceed through the cell cycle at the same rate as the other proliferative basal cells. However, their exact cell-cycle profile has not been determined because a pure population of ESCs has not been available. In this study, we sorted stem and transient amplifying (TA) cells from murine neonatal back skin, and adult ear, footpad, and back skin, using our recently developed method. We found that neonatal back skin had two times the number of ESCs as the adult tissues. Despite the age and anatomical difference, these ESC populations exhibited similar cell cycle profiles with approximately 96% in G0/G1 and 4% in S-G2/M. The cell cycle profiles of the TA cells from neonatal back skin and adult footpad also showed a profile similar to each other (85% in G1 and 15% in S-G2/M). Examination of genes on a cell cycle chip showed that proliferation associated genes and only p57 were upregulated in the TA cell and ESC population, respectively. We found BrdU positive and cyclin B1 positive cells in all groups, confirming that both ESCs and TA cells were cycling. These data demonstrate that there are more TA cells dividing than ESCs, that the cell cycle profile of adult TA cells is related to the proliferative state of the tissue in which they reside, and that ESC proceed through the cell cycle.

Animals, Newborn

Cell Cycle - genetics

Epidermis - metabolism

G2 Phase - genetics

Bromodeoxyuridine

Cyclin-Dependent Kinase Inhibitor p57

Stem Cells - cytology

Mitosis - genetics

Stem Cells - metabolism

Epidermis - growth & development

S Phase - genetics

Cyclin B1

Mice, Inbred ICR

Cell Differentiation - genetics

Animals

Cyclin B - genetics

Flow Cytometry

Resting Phase, Cell Cycle - genetics

Aging - genetics

Mice

G1 Phase - genetics

Nuclear Proteins - genetics

Epidermis - cytology

Details

Title: Subtitle: Mouse epidermal stem cells proceed through the cell cycle
Creators: Martine Dunnwald - Department of Anatomy and Cell Biology, The University of Iowa, Iowa City, Iowa, USA. martine-dunnwald@uiowa.edu
Sathivel Chinnathambi
Dana Alexandrunas
Jackie R Bickenbach
Resource Type: Journal article
Publication Details: Journal of cellular physiology, Vol.195(2), pp.194-201
DOI: 10.1002/jcp.10311
PMID: 12652646
NLM abbreviation: J Cell Physiol
ISSN: 0021-9541
eISSN: 1097-4652
Publisher: United States
Grant note: R01-AR45259 / NIAMS NIH HHS R01-DE13006 / NIDCR NIH HHS
Language: English
Date published: 05/2003
Academic Unit: Dermatology; Anatomy and Cell Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984025467502771

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