Journal article
Multi-omics inhalation toxicity assessment of urban soil dusts contaminated by multiple legacy sources of lead (Pb)
Journal of hazardous materials, Vol.480, 136120
10/09/2024
DOI: 10.1016/j.jhazmat.2024.136120
PMCID: PMC11698073
PMID: 39405708
Abstract
Although animal studies have evaluated lead (Pb) toxicity, they are limited to soluble forms, such as Pb-acetate, which do not reflect the range found in the exposome. Recent studies on Pb speciation of residential soils in urban areas revealed that the initial Pb sources are not persistent and are extensively repartitioned into adsorbed forms of Pb rather than insoluble phosphates. We investigated the inhalation and neurological toxicity of dusts generated from a surficial soil sample collected from a residential site with an exposomic mixture of various Pb species, both adsorbed phases (Fe and Mn oxide, humate bound Pb) and mineral phases (Pb hydroxycarbonate, pyromorphite, galena). Mice inhaled East Chicago dust (ECD) generated from a composite soil sample for 4 h/day, 7 days/week, for 4 weeks. Mice were necropsied immediately, 1, 14 and 30 days post exposure to evaluate both toxicity and recovery. Exposure to ECD caused changes in memory and spatial learning in the Morris Water Maze test. RNAseq analysis of the hippocampus region revealed multiple differentially expressed genes and impacts on pathways involved in ion channel complexes, and neuron-to-neuron synapse. Metabolomics analysis of plasma highlighted significant alterations in metabolic processes immediately after exposure that resolved after 14 days of rest.
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•PM generated from residential soils contaminated by multiple legacy Pb sources.•Mice were exposed in a nose-only system for 4 h/day, 7 days/week, for 4 weeks.•Exposure caused changes in memory and spatial learning in Morris Water Maze test.•RNAseq of hippocampus revealed differentially expressed genes and impacted pathways.•Metabolomics of plasma highlighted significant alterations in metabolic processes.
Details
- Title: Subtitle
- Multi-omics inhalation toxicity assessment of urban soil dusts contaminated by multiple legacy sources of lead (Pb)
- Creators
- Ezazul Haque - University of IowaAndrea Adamcakova-Dodd - University of IowaXuefang Jing - University of IowaHui Wang - University of IowaAlan K. Jarmusch - National Institutes of HealthPeter S. Thorne - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of hazardous materials, Vol.480, 136120
- DOI
- 10.1016/j.jhazmat.2024.136120
- PMID
- 39405708
- PMCID
- PMC11698073
- NLM abbreviation
- J Hazard Mater
- ISSN
- 0304-3894
- eISSN
- 1873-3336
- Publisher
- Elsevier B.V
- Grant note
- University of Iowa's Environmental Health Sciences Research Center: NIH P30ES005605 NIH: P42ES013661 Intramural Research Program of the NIH, National Institute of Environmental Health Sciences: ZO1 ES25045
We thank Dr. Hanna Stevens for her expertise in neurobehavioral testing, Dr. Drew Latta for ICP-MS related technical assistance. We also thank Vingie Ng, Casey Pulliam, and Laura Dean for assisting with necropsies and Dr. Benjamin Bostick for manuscript review. The authors thank the members of the East Chicago Community Advisory Group (CAG), and Sarah Rolfes from US EPA Region 5 for their assistance in engagement with the East Chicago community. This work was supported by the University of Iowa's Environmental Health Sciences Research Center, grant NIH P30ES005605. We acknowledge support for the AESOP Study from NIH P42ES013661 for related work on environmental exposures to legacy pollutants in East Chicago. E.H. is supported by the Intramural Research Program of the NIH, National Institute of Environmental Health Sciences (ZO1 ES25045).
- Language
- English
- Date published
- 10/09/2024
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health
- Record Identifier
- 9984736736902771
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