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Multi-site DMS probing reveals higher-order structure of RNA-protein complexes in living cells
Journal article   Open access   Peer reviewed

Multi-site DMS probing reveals higher-order structure of RNA-protein complexes in living cells

Irfana Saleem, Thomas Miller, Lucas Kearns, Anthony Hoang, Joshua Meehan, Ritwika Bose, David Mitchell, David H. Price, Calla M. Olson, Chase A. Weidmann, …
Molecular cell, Vol.86(9), pp.1815-1830.e9
05/2026
DOI: 10.1016/j.molcel.2026.03.029
PMCID: PMC13120737
PMID: 42019497
url
https://doi.org/10.1016/j.molcel.2026.03.029View
Published (Version of record) Open Access

Abstract

Identifying tertiary structures and protein binding sites in RNA molecules remains a key challenge in RNA biology. We describe multi-site dimethyl sulfate (DMS)-mutational profiling (MaP) (msDMS-MaP), a strategy that enables simultaneous measurement of RNA secondary, tertiary, and quaternary structures via a single DMS chemical probing experiment. Optimized reverse transcription decodes typically invisible DMS N7-methylguanine (N7-G) modifications via a tautomer-induced mutational signature concurrent with N1 and N3 modifications. We show that N7-G reactivity reports on higher-order RNA structures, revealing key functional motifs such as pseudoknots and protein binding sites. Using msDMS-MaP, we find that E. coli ribosomal RNAs encode numerous independently folding tertiary structures that coincide with binding sites for primary assembly proteins. We further apply msDMS-MaP to define the quaternary structural ensemble of the 7SK small nuclear ribonucleoprotein particle (snRNP), revealing that each of the three 7SK structural isoforms possesses distinct protein binding profiles in cells. msDMS-MaP represents a broadly applicable strategy for enhanced RNA functional motif discovery and characterization. [Display omitted] •msDMS-MaP measures RNA secondary and higher-order structures in a single experiment•N7-G DMS reactivity specifically reports on RNA tertiary folding and protein binding•msDMS-MaP reveals protein-independent tertiary folding in ribosomal RNAs•7SK RNA structural remodeling is connected to changes in protein binding in cells Saleem et al. introduce multi-site DMS-MaP (msDMS-MaP), a chemical probing strategy that permits simultaneous mapping of RNA secondary structures and typically concealed tertiary structures and protein binding sites. msDMS-MaP broadly enables improved functional motif discovery and provides insights into ribosome assembly and the structure of the 7SK RNA.
 7SK ribosome assembly RNA binding proteins RNA folding RNA motif discovery RNA structure RNA tertiary structure RNP structure modeling

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