Logo image
Back
Multicenter Evaluation of Use of Dried Blood and Plasma Spot Specimens in Quantitative Assays for Human Immunodeficiency Virus RNA: Measurement, Precision, and RNA Stability
Journal article   Open access   Peer reviewed

Multicenter Evaluation of Use of Dried Blood and Plasma Spot Specimens in Quantitative Assays for Human Immunodeficiency Virus RNA: Measurement, Precision, and RNA Stability

Don Brambilla, Cheryl Jennings, Grace Aldrovandi, James Bremer, Anne Marie Comeau, Sharon A Cassol, Ruth Dickover, J. Brooks Jackson, Jane Pitt, John L Sullivan, …
Journal of clinical microbiology, Vol.41(5), pp.1888-1893
05/2003
DOI: 10.1128/JCM.41.5.1888-1893.2003
PMCID: PMC154666
PMID: 12734222
url
https://doi.org/10.1128/JCM.41.5.1888-1893.2003View
Published (Version of record) Open Access

Abstract

Eleven laboratories evaluated the use of dried blood and plasma spots for quantitation of human immunodeficiency virus (HIV) RNA by two commercially available RNA assays, the Roche Amplicor HIV-1 Monitor and the bioMerieux NucliSens HIV-1 QT assays. The recovery of HIV RNA was linear over a dynamic range extending from 4,000 to 500,000 HIV type 1 RNA copies/ml. The Monitor assay appeared to have a broader dynamic range and seemed more sensitive at lower concentrations. However, the NucliSens assay gave more consistent results and could be performed without modification of the kit. HIV RNA was stable in dried whole blood or plasma stored at room temperature or at −70°C for up to 1 year. Dried blood and dried plasma spots can be used as an easy and inexpensive means for the collection and storage of specimens under field conditions for the diagnosis of HIV infection and the monitoring of antiretroviral therapy.
 Virology

Details

Metrics

18 Record Views
127 Times Cited - Web of Science
Logo image