Multicenter evaluation of quantification methods for plasma human immunodeficiency virus type 1 RNA

Hsiang Ju Lin; Lawrence E Myers; Belinda Yen-Lieberman; F Blaine Hollinger; Denis Henrard; Carol J Hooper; Robert Kokka; Shirley Kwok; Suraiya Rasheed; Maryanne Vahey; Mark A Winters; Lisa J McQuay; Peter L Nara; Patricia Reichelderfer; Robert W Coombs; J Brooks Jackson

doi:10.1093/infdis/170.3.553

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Multicenter evaluation of quantification methods for plasma human immunodeficiency virus type 1 RNA

Journal article

Peer reviewed

Multicenter evaluation of quantification methods for plasma human immunodeficiency virus type 1 RNA

Hsiang Ju Lin, Lawrence E Myers, Belinda Yen-Lieberman, F Blaine Hollinger, Denis Henrard, Carol J Hooper, Robert Kokka, Shirley Kwok, Suraiya Rasheed, Maryanne Vahey, …

The Journal of infectious diseases, Vol.170(3), pp.553-562

09/1994

DOI: 10.1093/infdis/170.3.553

PMID: 7915748

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Abstract

Six procedures for quantifying plasma human immunodeficiency virus type 1 (HIV-1) RNA were evaluated by nine laboratories. The procedures differed in their sample volume and preparation of samples and methods of amplification and detection. Coded samples in a 10-fold dilution series of HIV-1-spiked plasma were correctly ranked by all six procedures. Subsequently, coded duplicate plasma samples from 16 HIV-1-infected patients were tested using a common set of standards. Several HIV-1 RNA procedures were sufficiently reproducible so that an empiric 4-fold change could be viewed as significant. HIV-1 RNA levels in the patients (up to 370,000 RNA copies/mL) correlated with proviral HIV-1 DNA and were inversely correlated with CD4 cell counts; HIV-1 RNA assays were more sensitive than plasma viremia, standard p24 antigen, or immune complex-dissociated p24 antigen assays. This study demonstrated that several HIV-1 RNA quantitative assays are ready for use in clinical trials.

Laboratories - standards

HIV Seronegativity

Predictive Value of Tests

HIV Seropositivity - blood

Humans

RNA, Viral - blood

HIV Seropositivity - diagnosis

Blood Specimen Collection

HIV-1 - isolation & purification

Sensitivity and Specificity

Acquired Immunodeficiency Syndrome - diagnosis

CD4-Positive T-Lymphocytes

Leukocyte Count

Acquired Immunodeficiency Syndrome - blood

HIV Core Protein p24 - blood

Viremia - blood

Details

Title: Subtitle: Multicenter evaluation of quantification methods for plasma human immunodeficiency virus type 1 RNA
Creators: Hsiang Ju Lin - Division of Molecular Virology, Baylor College of Medicine, Houston, Texas
Lawrence E Myers
Belinda Yen-Lieberman
F Blaine Hollinger
Denis Henrard
Carol J Hooper
Robert Kokka
Shirley Kwok
Suraiya Rasheed
Maryanne Vahey
Mark A Winters
Lisa J McQuay
Peter L Nara
Patricia Reichelderfer
Robert W Coombs
J Brooks Jackson - University of Iowa, Pathology
Resource Type: Journal article
Publication Details: The Journal of infectious diseases, Vol.170(3), pp.553-562
Publisher: United States
DOI: 10.1093/infdis/170.3.553
PMID: 7915748
ISSN: 0022-1899
eISSN: 1537-6613
Grant note: AI-82517 / NIAID NIH HHS AI-25879 / NIAID NIH HHS AI-27664 / NIAID NIH HHS
Language: English
Date published: 09/1994
Academic Unit: Pathology; VPMA - Administration
Record Identifier: 9984047871502771

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