Multifunctional nanorods for gene delivery

Aliasger K Salem; Peter C Searson; Kam W Leong

doi:10.1038/nmat974

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Multifunctional nanorods for gene delivery

Journal article

Peer reviewed

Multifunctional nanorods for gene delivery

Aliasger K Salem, Peter C Searson and Kam W Leong

Nature materials, Vol.2(10), pp.668-671

10/2003

DOI: 10.1038/nmat974

PMID: 12970757

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Abstract

The goal of gene therapy is to introduce foreign genes into somatic cells to supplement defective genes or provide additional biological functions, and can be achieved using either viral or synthetic non-viral delivery systems. Compared with viral vectors, synthetic gene-delivery systems, such as liposomes and polymers, offer several advantages including ease of production and reduced risk of cytotoxicity and immunogenicity, but their use has been limited by the relatively low transfection efficiency. This problem mainly stems from the difficulty in controlling their properties at the nanoscale. Synthetic inorganic gene carriers have received limited attention in the gene-therapy community, the only notable example being gold nanoparticles with surface-immobilized DNA applied to intradermal genetic immunization by particle bombardment. Here we present a non-viral gene-delivery system based on multisegment bimetallic nanorods that can simultaneously bind compacted DNA plasmids and targeting ligands in a spatially defined manner. This approach allows precise control of composition, size and multifunctionality of the gene-delivery system. Transfection experiments performed in vitro and in vivo provide promising results that suggest potential in genetic vaccination applications.

Cells, Cultured

Drug Carriers - administration & dosage

Drug Carriers - chemical synthesis

Drug Delivery Systems - instrumentation

Drug Delivery Systems - methods

Equipment Design

Equipment Failure Analysis

Genetic Therapy - instrumentation

Genetic Therapy - methods

Humans

Kidney - cytology

Kidney - drug effects

Kidney - embryology

Kidney - metabolism

Nanotechnology - instrumentation

Nanotechnology - methods

Plasmids - administration & dosage

Plasmids - genetics

Plasmids - pharmacokinetics

Transfection - instrumentation

Transfection - methods

Transferrin - administration & dosage

Transferrin - pharmacokinetics

Details

Title: Subtitle: Multifunctional nanorods for gene delivery
Creators: Aliasger K Salem - Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
Peter C Searson
Kam W Leong
Resource Type: Journal article
Publication Details: Nature materials, Vol.2(10), pp.668-671
DOI: 10.1038/nmat974
PMID: 12970757
NLM abbreviation: Nat Mater
ISSN: 1476-1122
eISSN: 1476-4660
Language: English
Date published: 10/2003
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
Record Identifier: 9984216724402771

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