Journal article
Multifunctional polymersomes for cytosolic delivery of gemcitabine and doxorubicin to cancer cells
Biomaterials, Vol.35(24), pp.6482-6497
08/2014
DOI: 10.1016/j.biomaterials.2014.04.026
PMCID: PMC4059508
PMID: 24797878
Abstract
Although liposomes are widely used as carriers of drugs and imaging agents, they suffer from a lack of stability and the slow release of the encapsulated contents at the targeted site. Polymersomes (vesicles of amphiphilic polymers) are considerably more stable compared to liposomes; however, they also demonstrate a slow release for the encapsulated contents, limiting their efficacy as a drug-delivery tool. As a solution, we prepared and characterized echogenic polymersomes, which are programmed to release the encapsulated drugs rapidly when incubated with cytosolic concentrations of glutathione. These vesicles encapsulated air bubbles inside and efficiently reflected diagnostic-frequency ultrasound. Folate-targeted polymersomes showed an enhanced uptake by breast and pancreatic-cancer cells in a monolayer as well as in three-dimensional spheroid cultures. Polymersomes encapsulated with the anticancer drugs gemcitabine and doxorubicin showed significant cytotoxicity to these cells. With further improvements, these vesicles hold the promise to serve as multifunctional nanocarriers, offering a triggered release as well as diagnostic ultrasound imaging.
Details
- Title: Subtitle
- Multifunctional polymersomes for cytosolic delivery of gemcitabine and doxorubicin to cancer cells
- Creators
- Rahul Nahire - North Dakota State UniversityManas K. Haldar - North Dakota State UniversityShirshendu Paul - University of DelawareAvinash H. Ambre - North Dakota State UniversityVarsha Meghnani - North Dakota State UniversityBuddhadev Layek - North Dakota State UniversityKalpana S. Katti - North Dakota State UniversityKara N. Gange - North Dakota State UniversityJagdish Singh - North Dakota State UniversityKausik Sarkar - Department of Mechanical Engineering, University of Delaware, Newark, DE 19716, United States; Department of Mechanical and Aerospace Engineering, George Washington University, Washington, DC 20052, United States.Sanku Mallik - North Dakota State University
- Resource Type
- Journal article
- Publication Details
- Biomaterials, Vol.35(24), pp.6482-6497
- DOI
- 10.1016/j.biomaterials.2014.04.026
- PMID
- 24797878
- PMCID
- PMC4059508
- NLM abbreviation
- Biomaterials
- ISSN
- 0142-9612
- eISSN
- 1878-5905
- Publisher
- Elsevier Ltd
- Grant note
- DOI: 10.13039/100000002, name: NIH, award: 1R01 CA 113746, P20RR016472; DOI: 10.13039/100000001, name: NSF, award: DMR 1005011, DMR 1306154, DMR 1005283
- Language
- English
- Date published
- 08/2014
- Academic Unit
- Orthopedics and Rehabilitation
- Record Identifier
- 9984304687002771
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