Multilocus Sequence Typing versus Pulsed-Field Gel Electrophoresis for Characterization of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates

Lucia L Nemoy; Mamuka Kotetishvili; Justine Tigno; Ananda Keefer-Norris; Anthony D Harris; Eli N Perencevich; Judith A Johnson; Dave Torpey; Alexander Sulakvelidze; J Glenn Morris Jr; O Colin Stine

doi:10.1128/JCM.43.4.1776-1781.2005

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Multilocus Sequence Typing versus Pulsed-Field Gel Electrophoresis for Characterization of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates

Journal article

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Peer reviewed

Multilocus Sequence Typing versus Pulsed-Field Gel Electrophoresis for Characterization of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates

Lucia L Nemoy, Mamuka Kotetishvili, Justine Tigno, Ananda Keefer-Norris, Anthony D Harris, Eli N Perencevich, Judith A Johnson, Dave Torpey, Alexander Sulakvelidze, J Glenn Morris Jr, …

Journal of clinical microbiology, Vol.43(4), pp.1776-1781

04/2005

DOI: 10.1128/JCM.43.4.1776-1781.2005

PMCID: PMC1081380

PMID: 15814998

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Abstract

Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains are emerging pathogens. Molecular typing of ESBL-producing E. coli is useful for surveillance purposes, to monitor outbreaks and track nosocomial spread. Although pulsed-field gel electrophoresis (PFGE) is the current "gold standard" for bacterial molecular typing, multilocus sequence typing (MLST) may offer advantages. Forty ESBL-producing E. coli isolates were selected at random from a cohort of intensive care unit patients who had active surveillance perirectal cultures done. PFGE identified 19 unique PFGE types (PT) among the 40 isolates; MLST identified 22 unique sequence types. MLST had greater discriminatory ability than PFGE for ESBL-producing E. coli. Simpson's indices of diversity for PFGE and MLST were 0.895 and 0.956, respectively. There were five clonal complexes (CCs) (isolates with differences of no more than two loci) that each contained multiple PT, but each PT was found in only one CC, indicating genetic consistency within a CC. MLST has clear utility in studies of ESBL-producing E. coli, based on a greater discriminatory ability and reproducibility than PFGE and the ability to a priori define genetically related bacterial strains.

Humans

Escherichia coli Infections - microbiology

Molecular Sequence Data

beta-Lactamases - biosynthesis

Electrophoresis, Gel, Pulsed-Field

Sequence Analysis, DNA

Escherichia coli Infections - epidemiology

beta-Lactamases - genetics

Escherichia coli - genetics

Escherichia coli - isolation & purification

Bacterial Typing Techniques

Cross Infection - microbiology

Escherichia coli Proteins - genetics

DNA, Bacterial - analysis

Cross Infection - epidemiology

Escherichia coli - classification

Details

Title: Subtitle: Multilocus Sequence Typing versus Pulsed-Field Gel Electrophoresis for Characterization of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates
Creators: Lucia L Nemoy - University of Maryland, Baltimore
Mamuka Kotetishvili
Justine Tigno
Ananda Keefer-Norris
Anthony D Harris
Eli N Perencevich
Judith A Johnson
Dave Torpey
Alexander Sulakvelidze
J Glenn Morris Jr
O Colin Stine
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.43(4), pp.1776-1781
DOI: 10.1128/JCM.43.4.1776-1781.2005
PMID: 15814998
PMCID: PMC1081380
NLM abbreviation: J Clin Microbiol
ISSN: 0095-1137
eISSN: 1098-660X
Publisher: United States
Grant note: 5K23AI001752-02 / NIAID NIH HHS K23 AI001752 / NIAID NIH HHS
Language: English
Date published: 04/2005
Academic Unit: Epidemiology; Internal Medicine
Record Identifier: 9983779295002771

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