Multimodality noninvasive imaging of gene transfer using the human sodium iodide symporter

Gang Niu; Andrew W Gaut; Laura L Boles Ponto; Richard D Hichwa; Mark T Madsen; Michael M Graham; Frederick E Domann

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Multimodality noninvasive imaging of gene transfer using the human sodium iodide symporter

Journal article

Peer reviewed

Multimodality noninvasive imaging of gene transfer using the human sodium iodide symporter

Gang Niu, Andrew W Gaut, Laura L Boles Ponto, Richard D Hichwa, Mark T Madsen, Michael M Graham and Frederick E Domann

The Journal of nuclear medicine (1978), Vol.45(3), pp.445-449

03/2004

PMID: 15001685

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Abstract

In this study we investigated the feasibility of using radionuclide accumulation mediated by the human sodium iodide symporter (hNIS) gene in conjunction with various imaging modalities as a reporter system to noninvasively monitor the expression of transgenes delivered for gene therapy. NIS-expressing adenovirus (Ad-hNIS) was delivered in vitro to MB-435 breast carcinoma cells. NIS-mediated accumulation of (125)I(-), (99m)TcO(4)(-), and (76)Br(-) by the cells was visualized using autoradiography, gamma-camera scintigraphy, and PET imaging, respectively. For all imaging modalities, signal intensity generated by the cells correlated linearly both with the amount of Ad-hNIS and with the activity of radionuclide added to the cells. hNIS-mediated cellular accumulation of radionuclide was clearly visualized by all 3 imaging modalities tested. This preliminary study demonstrates the feasibility of using hNIS for monitoring the location and magnitude of expression of genes delivered during gene therapy.

Gene Transfer Techniques

Radiopharmaceuticals - pharmacokinetics

Humans

Gene Expression Regulation, Neoplastic

Breast Neoplasms - metabolism

Feasibility Studies

Genes, Reporter - physiology

Recombinant Fusion Proteins - pharmacokinetics

Radionuclide Imaging

Cell Line, Tumor

Breast Neoplasms - diagnostic imaging

Symporters - pharmacokinetics

Genetic Therapy - methods

Details

Title: Subtitle: Multimodality noninvasive imaging of gene transfer using the human sodium iodide symporter
Creators: Gang Niu - Free Radical and Radiation Biology Program, Department of Radiation Oncology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, USA
Andrew W Gaut
Laura L Boles Ponto
Richard D Hichwa
Mark T Madsen
Michael M Graham
Frederick E Domann
Resource Type: Journal article
Publication Details: The Journal of nuclear medicine (1978), Vol.45(3), pp.445-449
Publisher: United States
PMID: 15001685
ISSN: 0161-5505
eISSN: 1535-5667
Grant note: R24 CA86307 / NCI NIH HHS CA91709 / NCI NIH HHS
Language: English
Date published: 03/2004
Academic Unit: Radiology; Research Administration; Pathology; Pharmaceutical Sciences and Experimental Therapeutics; Surgery; Physics and Astronomy; Radiation Oncology
Record Identifier: 9984046814102771

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