Multiparameter Flow Cytometric Analysis Reveals Low Percentage of Bone Marrow Hematogones in Myelodysplastic Syndromes

Sepideh MAFTOUN-BANANKHAH; Atousa MALEKI; Nitin J KARANDIKAR; Arnaldo A ARBINI; Franklin S FUDA; Huan-You WANG; Weina Chen

doi:10.1309/4W2G3NDXUPG5J33N

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Multiparameter Flow Cytometric Analysis Reveals Low Percentage of Bone Marrow Hematogones in Myelodysplastic Syndromes

Journal article

Open access

Peer reviewed

Multiparameter Flow Cytometric Analysis Reveals Low Percentage of Bone Marrow Hematogones in Myelodysplastic Syndromes

Sepideh MAFTOUN-BANANKHAH, Atousa MALEKI, Nitin J KARANDIKAR, Arnaldo A ARBINI, Franklin S FUDA, Huan-You WANG and Weina Chen

American journal of clinical pathology, Vol.129(2), pp.300-308

2008

DOI: 10.1309/4W2G3NDXUPG5J33N

PMID: 18208811

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Abstract

Diagnosis of myelodysplastic syndromes (MDS) could be difficult. We explored the usefulness of the enumeration of maturing B-lineage precursors (hematogones) by multiparameter flow cytometric analysis in the diagnosis of MDS in bone marrow (BM) specimens. We evaluated 111 MDS, 120 non-MDS (most with cytopenias; control group 1), and 41 noncytopenic lymphoma staging BM (control group 2) specimens. The percentage of total hematogones was significantly lower in MDS (median, 0%; mean, 0.10%) compared with non-MDS (control group 1, median, 0.38%, and mean, 0.91%; control group 2, median, 0.38%, and mean, 0.60%; P < .0001), as was the percentage of the most immature (stage I) hematogones. Thus, hematogone enumeration may serve as a biomarker to aid in the diagnosis of MDS. Interestingly, the percentage of hematogones was not significantly different between MDS subgroups or patients with MDS with and without chromosomal abnormalities, implying that a defect in maturing B-cell precursors may be an early event in the pathogenesis of MDS.

Hematologic and hematopoietic diseases

Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis

Biological and medical sciences

Investigative techniques, diagnostic techniques (general aspects)

Medical sciences

Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques

Details

Title: Subtitle: Multiparameter Flow Cytometric Analysis Reveals Low Percentage of Bone Marrow Hematogones in Myelodysplastic Syndromes
Creators: Sepideh MAFTOUN-BANANKHAH - Department of Pathology, University of Texas Southwestern Medical Center, Dallas, United States
Atousa MALEKI - Department of Pathology, University of Texas Southwestern Medical Center, Dallas, United States
Nitin J KARANDIKAR - Department of Pathology, University of Texas Southwestern Medical Center, Dallas, United States
Arnaldo A ARBINI - Department of Pathology, University of Texas Southwestern Medical Center, Dallas, United States
Franklin S FUDA - Department of Pathology, University of Texas Southwestern Medical Center, Dallas, United States
Huan-You WANG - Department of Pathology, University of Texas Southwestern Medical Center, Dallas, United States
Weina Chen - Department of Pathology, University of Texas Southwestern Medical Center, Dallas, United States
Resource Type: Journal article
Publication Details: American journal of clinical pathology, Vol.129(2), pp.300-308
Publisher: American Society of Clinical Pathologists; Chicago, IL
DOI: 10.1309/4W2G3NDXUPG5J33N
PMID: 18208811
ISSN: 0002-9173
eISSN: 1943-7722
Language: English
Date published: 2008
Academic Unit: Pathology
Record Identifier: 9984047867402771

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