Journal article
Multiparameter Flow Cytometric Assays to Quantify Effector and Regulatory T-Cell Function in Multiple Sclerosis
Journal of multiple sclerosis, Vol.2(1), 1000130
01/2015
DOI: 10.4172/2376-0389.1000130
PMCID: PMC4484619
PMID: 26137595
Abstract
The immune system plays a major pathological and regulatory role in multiple sclerosis (MS) and, therefore, is a focus of extensive research. Animal models of MS have been crucial in understanding the pathological processes in MS and developing certain treatments, however, all crucial aspects of the human disease may not be appropriately modeled. With the exception of detecting oligoclonal bands and IgG synthesis in cerebrospinal fluids of MS patients, there has not been major progress in the development of immunologic tests that can be used for diagnosis of MS. Further, due to the lack of validated immune assays, routine monitoring of the immune system following therapy initiation is not a part of standard patient care in MS. This is critical since immunomodulatory therapies used for MS treatment are not benign and, more importantly, there is a considerable variation in clinical responses in MS patients initiating such therapies. Flow cytometry is a powerful tool that can be used for studying both the phenotype and function of immune cells. The studies described here will demonstrate how flow cytometry can be used to apply current knowledge about the MS immune system to develop a diagnostic laboratory test for the immunologic monitoring of this disease. Importantly, we will also show that the multiparameter flow cytometry based assay developed by us can also be implemented for the immunologic evaluation of therapeutic success in MS patients.
Details
- Title: Subtitle
- Multiparameter Flow Cytometric Assays to Quantify Effector and Regulatory T-Cell Function in Multiple Sclerosis
- Creators
- Sushmita Sinha - Department of Pathology, University of Iowa, USAMichael P Crawford - Department of Pathology, University of Iowa, USASterling B Ortega - Department of Pathology, University of Iowa, USANitin J Karandikar - Department of Pathology, University of Iowa, USA
- Resource Type
- Journal article
- Publication Details
- Journal of multiple sclerosis, Vol.2(1), 1000130
- DOI
- 10.4172/2376-0389.1000130
- PMID
- 26137595
- PMCID
- PMC4484619
- NLM abbreviation
- J Mult Scler (Foster City)
- ISSN
- 2376-0389
- eISSN
- 2376-0389
- Publisher
- United States
- Grant note
- R01 AI065463 / NIAID NIH HHS K08 AI049990 / NIAID NIH HHS K24 AI079272 / NIAID NIH HHS R01 AI053439 / NIAID NIH HHS
- Language
- English
- Date published
- 01/2015
- Academic Unit
- Pathology
- Record Identifier
- 9984047690402771
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