Journal article
Multiple Pools of Nuclear Actin
Anatomical record (Hoboken, N.J. : 2007), Vol.301(12), pp.2014-2036
12/2018
DOI: 10.1002/ar.23964
PMCID: PMC6293971
PMID: 30312534
Abstract
ABSTRACT
While nuclear actin was reported ~50 years ago, it's in vivo prevalence and structure remain largely unknown. Here, we use Drosophila oogenesis, that is, follicle development, to characterize nuclear actin. We find that three different reagents—DNase I, anti‐actin C4, and anti‐actin AC15—recognize distinct pools of nuclear actin. DNase I labels monomeric or G‐actin, and, during follicle development, G‐actin is present in the nucleus of every cell. Some G‐actin is recognized by the C4 antibody. In particular, C4 nuclear actin colocalizes with DNase I to the nucleolus in anterior escort cells, follicle stem cells, some mitotic follicle cells, and a subset of nurse cells during early oogenesis. C4 also labels polymeric nuclear actin in the nucleoplasm of the germline stem cells, early cystoblasts, and oocytes. The AC15 antibody labels a completely distinct pool of nuclear actin from that of DNase I and C4. Specifically, AC15 nuclear actin localizes to the chromatin in the nurse and follicle cells during mid‐to‐late oogenesis. Within the oocyte, AC15 nuclear actin progresses from localizing to puncta surrounding the DNA, to forming a filamentous cage around the chromosomes. Together these findings reveal that nuclear actin is highly prevalent in vivo, and multiple pools of nuclear actin exist and can be recognized using different reagents. Additionally, our localization studies suggest that nuclear actin may regulate stemness, nucleolar structure and function, transcription, and nuclear structure. Such findings call for further studies to explore the prevalence, diversity, and functions of nuclear actin across tissues and organisms. Anat Rec, 301:2014–2036, 2018. © 2018 Wiley Periodicals, Inc.
Details
- Title: Subtitle
- Multiple Pools of Nuclear Actin
- Creators
- Dylane M Wineland - University of Iowa Carver College of MedicineDaniel J Kelpsch - University of Iowa Carver College of MedicineTina L Tootle - University of Iowa Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- Anatomical record (Hoboken, N.J. : 2007), Vol.301(12), pp.2014-2036
- Publisher
- John Wiley & Sons, Inc; Hoboken, USA
- DOI
- 10.1002/ar.23964
- PMID
- 30312534
- PMCID
- PMC6293971
- ISSN
- 1932-8486
- eISSN
- 1932-8494
- Number of pages
- 23
- Grant note
- National Institute of Neurological Disorders and Stroke (T32NS045549) National Institute of General Medical Sciences (R01GM116885)
- Language
- English
- Date published
- 12/2018
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984025444202771
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