Journal article
Multiple cis-acting sequence elements are required for efficient splicing of simian virus 40 small-t antigen pre-mRNA
Molecular and cellular biology, Vol.8(9), pp.3582-3590
09/1988
DOI: 10.1128/mcb.8.9.3582-3590.1988
PMCID: PMC365413
PMID: 2851720
Abstract
We have determined the effects of a number of mutations in the small-t antigen mRNA intron on the alternative splicing pattern of the simian virus 40 early transcript. Expansion of the distance separating the small-t pre-mRNA lariat branch point and the shared large T-small t 3' splice site from 18 to 29 nucleotides (nt) resulted in a relative enhancement of small-t splicing in vivo. This finding, coupled with the observation that large-T pre-RNA splicing in vitro was not affected by this expansion, suggests that small-t splicing is specifically constrained by a short branch point-3' splice site distance. Similarly, the distance separating the 5' splice site and branch point (48 nt) was found to be at or near a minimum for small-t splicing, because deletions in this region as small as 2 nt dramatically reduced the ratio of small-t to large-T mRNA that accumulated in transfected cells. Finally, a specific sequence within the small-t intron, encompassing the upstream branch sites used in large-T splicing, was found to be an important element in the cell-specific pattern of early alternative splicing. Substitutions within this region reduced the ratio of small-t to large-T mRNA produced in HeLa cells but had only minor effects in human 293 cells.
Details
- Title: Subtitle
- Multiple cis-acting sequence elements are required for efficient splicing of simian virus 40 small-t antigen pre-mRNA
- Creators
- Xin-Yuan Fu - Department of Biological Sciences, Columbia University, New York, New York 10027John D Colgan - Department of Biological Sciences, Columbia University, New York, New York 10027James L Manley - Department of Biological Sciences, Columbia University, New York, New York 10027
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular biology, Vol.8(9), pp.3582-3590
- DOI
- 10.1128/mcb.8.9.3582-3590.1988
- PMID
- 2851720
- PMCID
- PMC365413
- ISSN
- 0270-7306
- eISSN
- 1098-5549
- Language
- English
- Date published
- 09/1988
- Academic Unit
- Anatomy and Cell Biology; Immunology; Internal Medicine
- Record Identifier
- 9984025572502771
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