Journal article
Multiple differentiation potentials of neonatal dura mater-derived cells
Neurosurgery, Vol.60(2), pp.346-352
02/01/2007
DOI: 10.1227/01.NEU.0000249278.72063.59
PMID: 17290186
Abstract
OBJECTIVE: The involvement of the dura mater in calvarial development and bone healing lead to a hypothesis that progenitor cells with multiple differentiation potentials exist within this tissue. The present study investigated the differentiation potentials of dura mater-derived cells by driving them into several cell-restricted lineages.
METHODS: Dissected dura mater tissue of neonatal rats was washed, finely minced, and enzymatically digested. The harvested cells were exposed to different differentiation (osteogenic, adipogenic, and chondrogenic) and basic media.
RESULTS: At defined time points, dura mater-derived cells were observed to differentiate into osteoblastic, adipoblastic, and chondroblastic cells, evidenced by specific biochemical staining. In addition, gene expressions of osteogenesis (alkaline phosphatase, osteocalcin, and osteopontin), chondrogenesis (collagen Type II and aggrecan core protein) and adipogenesis (peroxisome proliferator activated receptor gamma-2) were up-regulated in the differentiated dura mater-derived cells, confirmed by polymerase chain reaction.
CONCLUSION: Preliminarily, it was concluded that a subpopulation of multiple potential mesenchymal cells exists in neonatal dura mater, which explains the function of the dura mater on neurocranium development and calvarial bone healing.
Details
- Title: Subtitle
- Multiple differentiation potentials of neonatal dura mater-derived cells
- Creators
- Ioana A. Peptan - University of Illinois ChicagoLiu Hong - University of Illinois ChicagoCarla A. Evans - University of Illinois Chicago
- Resource Type
- Journal article
- Publication Details
- Neurosurgery, Vol.60(2), pp.346-352
- DOI
- 10.1227/01.NEU.0000249278.72063.59
- PMID
- 17290186
- NLM abbreviation
- Neurosurgery
- ISSN
- 0148-396X
- eISSN
- 1524-4040
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 7
- Language
- English
- Date published
- 02/01/2007
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984367652202771
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