Multiple glucan-binding proteins of Streptococcus sobrinus

Y Ma; M O Lassiter; J A Banas; M Y Galperín; K G Taylor; R J Doyle

doi:10.1128/jb.178.6.1572-1577.1996

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Multiple glucan-binding proteins of Streptococcus sobrinus

Journal article

Open access

Peer reviewed

Multiple glucan-binding proteins of Streptococcus sobrinus

Y Ma, M O Lassiter, J A Banas, M Y Galperín, K G Taylor and R J Doyle

Journal of bacteriology, Vol.178(6), pp.1572-1577

03/1996

DOI: 10.1128/jb.178.6.1572-1577.1996

PMCID: PMC177840

PMID: 8626283

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Abstract

Several proteins from culture supernatants of Streptococcus sobrinus were able to bind avidly to Sephadex G-75. The proteins could be partially eluted from the Sephadex by low-molecular-weight alpha-1,6 glucan or fully eluted by 4 M guanidine hydrochloride. Elution profiles were complex, yielding proteins of 16, 45, 58 to 60, 90, 135, and 145 kDa, showing that the wild-type strain possessed multiple glucan-binding proteins. Two mutants of Streptococcus sobrinus incapable of aggregation by high-molecular-weight alpha-1,6 glucan were isolated. One mutant was spontaneous, from a cell suspension to which glucan had been added, whereas the other was induced by ethyl methanesulfonate. Both mutants were devoid of a 60-kDa protein, as shown by gel electrophoresis of culture supernatants and whole cells. Amino acid analysis showed that the 58- to 60-kDa protein and the 90-kDa protein were distinct, although both were N-terminally blocked. Both mutants retained their ability to adhere to glass in the presence of sucrose and to ferment mannitol and sorbitol. Both mutants retained their glucosytransferase activities, as shown by activity gels. Western blots (immunoblots), employing antibody against a glucan-binding protein of Streptococcus mutans, failed to reveal cross-reactivity with S. sobrinus proteins. The results show that even though S. sobrinus produces several proteins capable of binding alpha-1,6 glucans, the 60-kDa protein is probably the lectin needed for glucan-dependent cellular aggregation.

Dextrans - metabolism

Membrane Proteins - isolation & purification

Streptococcus sobrinus - chemistry

Membrane Proteins - genetics

Electrophoresis, Polyacrylamide Gel

Amino Acids - analysis

Bacterial Proteins - genetics

Streptococcus sobrinus - genetics

Blotting, Western

Bacterial Adhesion

Chromatography, Affinity

Bacterial Proteins - metabolism

Glucans - metabolism

Membrane Proteins - metabolism

Bacterial Proteins - isolation & purification

Details

Title: Subtitle: Multiple glucan-binding proteins of Streptococcus sobrinus
Creators: Y Ma - Department of Microbiology and Immunology, Academic Health Center, University of Louisville, Kentucky 40292, USA
M O Lassiter
J A Banas
M Y Galperín
K G Taylor
R J Doyle
Resource Type: Journal article
Publication Details: Journal of bacteriology, Vol.178(6), pp.1572-1577
DOI: 10.1128/jb.178.6.1572-1577.1996
PMID: 8626283
PMCID: PMC177840
NLM abbreviation: J Bacteriol
ISSN: 0021-9193
eISSN: 1098-5530
Publisher: United States
Grant note: DE-05102 / NIDCR NIH HHS DE-10058 / NIDCR NIH HHS DE-07199 / NIDCR NIH HHS
Language: English
Date published: 03/1996
Academic Unit: Pediatric Dentistry; Dental Research
Record Identifier: 9984066085702771

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