Journal article
Multiplex Microfluidic Paper-based Immunoassay for the Diagnosis of Hepatitis C Virus Infection
Analytical chemistry (Washington), Vol.86(11), pp.5338-5344
06/03/2014
DOI: 10.1021/ac500247f
PMID: 24794788
Abstract
Hepatitis C virus (HCV) infection is a serious and rising global healthcare problem. One critical challenge to tackle this disease is the lack of adequate diagnosis. Here, we develop a multiplex microfluidic paper-based immunoassay, as a novel diagnostic approach, to detect human IgG antibody against HCV (anti-HCV). The paper substrate, highly flammable nitrocellulose (NC), is patterned under ambient temperature by craft punch patterning (CPP) to generate multiple test zones. On the basis of superior merits of patterned paper, this new diagnostic approach demonstrates the key novelty to unprecedentedly combine segmented diagnostic assays into a single multiplex test. The generated diagnostic results are not only informative but can be rapidly and cost-effectively delivered. It would significantly transform the clinical pathway for unwitting individuals with HCV infection. This work highlights the promising role of microfluidic paper-based immunoassays in tackling the diagnostic challenge for the HCV pandemic as well as other diseases.
Details
- Title: Subtitle
- Multiplex Microfluidic Paper-based Immunoassay for the Diagnosis of Hepatitis C Virus Infection
- Creators
- Xuan Mu - Chinese Academy of Medical Sciences & Peking Union Medical CollegeLin Zhang - Peking Union Medical College HospitalShaoying Chang - Chinese Academy of Medical Sciences & Peking Union Medical CollegeWei Cui - Peking Union Medical College HospitalZhi Zheng - Chinese Academy of Medical Sciences & Peking Union Medical College
- Resource Type
- Journal article
- Publication Details
- Analytical chemistry (Washington), Vol.86(11), pp.5338-5344
- Publisher
- Amer Chemical Soc
- DOI
- 10.1021/ac500247f
- PMID
- 24794788
- ISSN
- 0003-2700
- eISSN
- 1520-6882
- Number of pages
- 7
- Grant note
- 2013ZX09507005 / National Scientific and Technological Major Project of China 21305162; 21375119; 81271926 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC)
- Language
- English
- Date published
- 06/03/2014
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9984276454302771
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