Journal article
Multiplex polymerase chain reaction
Modern pathology, Vol.5(3), pp.320-323
05/1992
PMID: 1323106
Abstract
The polymerase chain reaction (PCR) is a widely utilized assay for specifically amplifying small fragments of DNA. Multiplex PCR is the amplification of more than one DNA fragment per reaction and has many potential uses. When more than one primer set per reaction tube is utilized, the total number of tubes in any one experiment may be reduced, conserving expensive reagents and decreasing possible contamination. Multiplex PCR allows for an assay of the gene of interest and assures that the amplification process proceeds as expected with the use of a companion control genome primer set. Multiplex PCR is useful in assaying DNA extracted from samples of immunocompromised patients in which more than one infectious agent may be suspected such as simultaneous EBV and CMV detection. Multiplex PCR offers many advantages over single reaction PCR and has been found to be an useful adjunct in our laboratory.
Details
- Title: Subtitle
- Multiplex polymerase chain reaction
- Creators
- L J Burgart - Department of Pathology, University of Iowa College of Medicine, Iowa CityR A RobinsonM J HellerW W WilkeO K IakoubovaJ C Cheville
- Resource Type
- Journal article
- Publication Details
- Modern pathology, Vol.5(3), pp.320-323
- Publisher
- United States
- PMID
- 1323106
- ISSN
- 0893-3952
- eISSN
- 1530-0285
- Language
- English
- Date published
- 05/1992
- Academic Unit
- Oral Pathology, Radiology and Medicine; Pathology
- Record Identifier
- 9984047615002771
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