Multipotent capacity of immortalized human bronchial epithelial cells

Oliver Delgado; Aadil A Kaisani; Monica Spinola; Xian-Jin Xie; Kimberly G Batten; John D Minna; Woodring E Wright; Jerry W Shay

doi:10.1371/journal.pone.0022023

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Multipotent capacity of immortalized human bronchial epithelial cells

Journal article

Open access

Peer reviewed

Multipotent capacity of immortalized human bronchial epithelial cells

Oliver Delgado, Aadil A Kaisani, Monica Spinola, Xian-Jin Xie, Kimberly G Batten, John D Minna, Woodring E Wright and Jerry W Shay

PloS one, Vol.6(7), e22023

2011

DOI: 10.1371/journal.pone.0022023

PMCID: PMC3131301

PMID: 21760947

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https://doi.org/10.1371/journal.pone.0022023View

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Abstract

While the adult murine lung utilizes multiple compartmentally restricted progenitor cells during homeostasis and repair, much less is known about the progenitor cells from the human lung. Translating the murine stem cell model to humans is hindered by anatomical differences between species. Here we show that human bronchial epithelial cells (HBECs) display characteristics of multipotent stem cells of the lung. These HBECs express markers indicative of several epithelial types of the adult lung when experimentally tested in cell culture. When cultured in three different three-dimensional (3D) systems, subtle changes in the microenvironment result in unique responses including the ability of HBECs to differentiate into multiple central and peripheral lung cell types. These new findings indicate that the adult human lung contains a multipotent progenitor cell whose differentiation potential is primarily dictated by the microenvironment. The HBEC system is not only important in understanding mechanisms for specific cell lineage differentiation, but also for examining changes that correlate with human lung diseases including lung cancer.

Biomarkers - metabolism

Tissue Culture Techniques

Humans

Cells, Cultured

Gene Expression Regulation

Epithelial Cells - ultrastructure

Gene Expression Profiling

Cyclin-Dependent Kinase 4 - metabolism

Cell Differentiation - genetics

Models, Biological

Multipotent Stem Cells - cytology

Pulmonary Alveoli - metabolism

Epithelial Cells - enzymology

Pulmonary Alveoli - cytology

Telomerase - metabolism

Epithelial Cells - cytology

Bronchi - cytology

Cell Line, Transformed

Details

Title: Subtitle: Multipotent capacity of immortalized human bronchial epithelial cells
Creators: Oliver Delgado - Department of Cell Biology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, United States of America
Aadil A Kaisani
Monica Spinola
Xian-Jin Xie
Kimberly G Batten
John D Minna
Woodring E Wright
Jerry W Shay
Resource Type: Journal article
Publication Details: PloS one, Vol.6(7), e22023
DOI: 10.1371/journal.pone.0022023
PMID: 21760947
PMCID: PMC3131301
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: United States
Grant note: P50 CA070907 / NCI NIH HHS P50CA70907 / NCI NIH HHS
Language: English
Date published: 2011
Academic Unit: Preventive and Community Dentistry; Biostatistics; Dental Research
Record Identifier: 9983917676702771

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