Journal article
Murine Astrocytes Express a Functional Chemokine Receptor
The Journal of neuroscience, Vol.17(17), pp.6522-6528
09/01/1997
DOI: 10.1523/JNEUROSCI.17-17-06522.1997
PMCID: PMC6573137
PMID: 9254664
Abstract
Elevated levels of chemokines have been observed in various diseases of the CNS. Little is known, however, about how these chemokines affect parenchymal cells of the CNS. The current studies examine astrocyte chemotaxis to the mouse chemokine macrophage inflammatory protein-1α (MIP-1α). Murine astrocytes demonstrate directed migration along a chemical gradient in response to 10
−10
–10
−8
m
MIP-1α. Peak chemotactic responses are noted at 10
−9
m
. MIP-1α-induced astrocyte migration is specifically inhibitable with pertussis toxin, suggesting a role for Gα
i
proteins in the signaling process.
RT-PCR and
in situ
hybridization were used to identify expression of the murine CCR1 MIP-1α receptor on astrocytes. Astrocytes contain mRNA for CCR1, but messages for CCR4 and the orphan chemokine receptor MIP-1αR-like#1 were not detected. The combined results suggest that a functional chemokine receptor is expressed on resident cells of the CNS. We speculate that the interactions of chemokines with astrocytes are involved in inflammatory reactions of the CNS.
Details
- Title: Subtitle
- Murine Astrocytes Express a Functional Chemokine Receptor
- Creators
- Shigeyuki Tanabe - Harvard UniversityMichael Heesen - Harvard UniversityMichael A. Berman - Harvard UniversityMichael B. Fischer - Harvard UniversityIzumi Yoshizawa - Harvard UniversityYi Luo - Harvard UniversityMartin E. Dorf - Harvard University
- Resource Type
- Journal article
- Publication Details
- The Journal of neuroscience, Vol.17(17), pp.6522-6528
- Publisher
- Society for Neuroscience
- DOI
- 10.1523/JNEUROSCI.17-17-06522.1997
- PMID
- 9254664
- PMCID
- PMC6573137
- ISSN
- 0270-6474
- eISSN
- 1529-2401
- Language
- English
- Date published
- 09/01/1997
- Academic Unit
- Urology
- Record Identifier
- 9984320855302771
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