Journal article
Murine B-Cell Subsets Defined by CD23
Methods (San Diego, Calif.), Vol.8(1), pp.3-10
08/1995
DOI: 10.1006/meth.1995.1039
Abstract
The B-cell compartment is composed of a complex mixture of distinct subsets. Three anatomic sites that are particularly rich in B-cell subpopulations are the bone marrow, spleen, and peritoneal cavity. Our laboratory has found CD23, the low-affinity lgE Fc receptor (FcϵRII), to be of value in discriminating a number of murine B-cell subsets, especially when used in combination with other B-cell markers. In the bone marrow, the site of maturation, the presence of CD23 specifically delineates mature recirculating B cells, whereas its absence denotes the maturing B-cell populations. In the spleen, CD23 is constitutively expressed on the follicular or mantle B cells and is absent on the marginal zone and immature B cells. These latter two populations can be distinguished by differing levels of the heat stable antigen (HSA). In the peritoneal cavity, B2 (conventional) B cells display the CD23 antigen whereas the B1 (CD5+/sister) subset does not. Additional studies examining the expression of CD43 (leukosialin) have found this antigen to serve as a reciprocal marker to CD23 in many cases. In the bone marrow, a pan anti-CD43 antibody detects those B cells that are CD23 negative, and in the spleen and peritoneal cavity, the majority of CD23 negative B 1 cells are CD43 positive. Together, these studies demonstrate the utility of CD23, CD43, HSA, and other markers to discriminate between B-cell subsets and allow for more precise purification and analysis of these populations.
Details
- Title: Subtitle
- Murine B-Cell Subsets Defined by CD23
- Creators
- Cory G Best - University of IowaJohn D Kemp - University of IowaThomas J Waldschmidt - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Methods (San Diego, Calif.), Vol.8(1), pp.3-10
- Publisher
- Elsevier Inc
- DOI
- 10.1006/meth.1995.1039
- ISSN
- 1046-2023
- eISSN
- 1095-9130
- Language
- English
- Date published
- 08/1995
- Academic Unit
- Pathology
- Record Identifier
- 9984186513602771
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