Journal article
Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, Vol.2020(162), pp.1-18
08/01/2020
DOI: 10.3791/61616
PMCID: PMC9280391
PMID: 32894272
Abstract
The murine excisional wound model has been used extensively to study each of the sequentially overlapping phases of wound healing: inflammation, proliferation and remodeling. Murine wounds have a histologically well-defined and easily recognizable wound bed over which these different phases of the healing process are measurable. Within the field, it is common to use an arbitrarily defined "middle" of the wound for histological analyses. However, wounds are a three-dimensional entity and often not histologically symmetrical, supporting the need for a well-defined and robust method of quantification to detect morphometric defects with a small effect size. In this protocol, we describe the procedure for creating bilateral, full-thickness excisional wounds in mice as well as a detailed instruction on how to measure morphometric parameters using an image processing program on select serial sections. The two-dimension measurements of wound length, epidermal length, epidermal area, and wound area are used in combination with the known distance between sections to extrapolate the three-dimension epidermal area covering the wound, overall wound area, epidermal volume and wound volume. Although this detailed histological analysis is more time and resource consuming than conventional analyses, its rigor increases the likelihood of detecting novel phenotypes in an inherently complex wound healing process.
Details
- Title: Subtitle
- Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis
- Creators
- Lindsey Rhea - University of IowaMartine Dunnwald - University of Iowa
- Resource Type
- Journal article
- Publication Details
- JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, Vol.2020(162), pp.1-18
- DOI
- 10.3791/61616
- PMID
- 32894272
- PMCID
- PMC9280391
- NLM abbreviation
- J Vis Exp
- ISSN
- 1940-087X
- eISSN
- 1940-087X
- Publisher
- Journal Of Visualized Experiments
- Number of pages
- 18
- Grant note
- AR067739 / NIH/NIAMS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS)
- Language
- English
- Date published
- 08/01/2020
- Academic Unit
- Anatomy and Cell Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984284356802771
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