Journal article
Murine epidermal side population possesses unique angiogenic properties
Experimental cell research, Vol.314(4), pp.720-728
2008
DOI: 10.1016/j.yexcr.2007.11.002
PMID: 18067890
Abstract
Total epidermal keratinocytes are a heterogeneous population of cells, including undifferentiated stem/progenitor cells (EpSPs) and their more differentiated progeny (Non-SP cells). Our previous
in vivo data showed that EpSPs enhanced blood flow restoration when injected into an ischemic limb, whereas Non-SP cells had no significant effect on
in vivo blood flow restoration. However, the cellular and molecular mechanisms of this observation remain largely unknown. Therefore, the aim of this study was to investigate the angiogenic properties of different epidermal subpopulations
in vitro and the mechanism by which EpSPs enhanced blood flow
in vivo. Using migration assay and capillary network formation, we show that EpSPs secrete higher levels of pro-angiogenic molecules compared to Non-SP cells, unsorted keratinocytes and fibroblasts
in vitro. Secretion of vascular endothelial growth factor (VEGF) was detected at higher levels in EpSP conditioned medium than the medium conditioned by other epidermal subpopulations and fibroblasts. Also, RT-PCR analyses revealed a unique angiogenic gene profile for EpSPs. Finally, gene array data indicate significant changes in angiogenic gene expression six days after cell injection in murine ischemic limbs. Therefore, we conclude that EpSPs possess unique angiogenic properties and that these cells may be indirectly responsible for the angiogenic response previously observed in our ischemic limb model.
Details
- Title: Subtitle
- Murine epidermal side population possesses unique angiogenic properties
- Creators
- Christopher C Oberley - The Department of Dermatology, The University of Iowa, Iowa City, IA 52242, USAFrancoise Gourronc - The Department of Dermatology, The University of Iowa, Iowa City, IA 52242, USAShinkai Hakimi - The Department of Dermatology, The University of Iowa, Iowa City, IA 52242, USAMargaret Riordan - The Department of Dermatology, The University of Iowa, Iowa City, IA 52242, USASarah Bronner - The Department of Dermatology, The University of Iowa, Iowa City, IA 52242, USAChunhua Jiao - The Department of Integrative Physiology, The University of Iowa, Iowa City, IA 52242, USAMartine Dunnwald - The Department of Dermatology, The University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Experimental cell research, Vol.314(4), pp.720-728
- DOI
- 10.1016/j.yexcr.2007.11.002
- PMID
- 18067890
- NLM abbreviation
- Exp Cell Res
- ISSN
- 0014-4827
- eISSN
- 1090-2422
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 2008
- Academic Unit
- Microbiology and Immunology; Anatomy and Cell Biology; Craniofacial Anomalies Research Center; Ophthalmology and Visual Sciences
- Record Identifier
- 9984025451602771
Metrics
22 Record Views