Journal article
Muscle ribosome detachment factor
Acta neurologica Scandinavica, Vol.64(2), pp.108-121
Received July 14, 1980, accepted February 23, 1981
08/1981
DOI: 10.1111/j.1600-0404.1981.tb04393.x
Abstract
A protein that detaches ribosomes from rough microsomal membranes (“detachment factor”) (DF) was isolated from the cytosol fraction of rat and human muscle. The procedure of isolation included differential centrifugation, precipitation with ammonium sulfate and column chromatography with Sephadex G‐100. The protein which is not completely homogenous, has a molecular weight of 50,000–60,000 daltons, is heat labile and has an optimum pH at 7.4–7.6. The DF activity of the protein is inhibited by soybean trypsin inhibitor (73%), pepstatin (67%), and leupeptin (42%), although no proteolysis could be measured. The DF activity was tested on muscle samples (rough microsomal membranes) obtained from Duchenne muscular dystrophy (DMD) patients (7 cases) and normal controls (16 cases). The yield of membrane bound ribosomes (MBR) obtained from muscle samples of DMD patients by extraction with DF is 5‐fold higher than from muscle samples of normal controls. The difference in MBR yield is not related to the type of DF. Similar values for MBR were obtained with rat and human (normal and dystrophic) DF. Ribosomal protein synthesis (RPS) with ribosomes extracted by DF showed values similar to the RPS of detergent extracted ribosomes. Our findings suggest the observed increase in membrane bound ribosomes in DMD probably results from increased levels of mRNAs coding for membrane and secretory proteins such as collagen. Copyright © 1981, Wiley Blackwell. All rights reserved
Details
- Title: Subtitle
- Muscle ribosome detachment factor
- Creators
- Victor Ionasescu - University of IowaSusana Braga - University of IowaLarry Kaeding - University of IowaPeter Rubenstein - University of IowaGeorge Kalnitsky - University of IowaRanjit Chatterjee - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Acta neurologica Scandinavica, Vol.64(2), pp.108-121
- Edition
- Received July 14, 1980, accepted February 23, 1981
- DOI
- 10.1111/j.1600-0404.1981.tb04393.x
- ISSN
- 0001-6314
- eISSN
- 1600-0404
- Publisher
- Blackwell Publishing Ltd
- Number of pages
- 14
- Language
- English
- Date published
- 08/1981
- Academic Unit
- Stead Family Department of Pediatrics; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984293083702771
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