Muscles of mice deficient in α-sarcoglycan maintain large masses and near control force values throughout the life span

Christina M Consolino; Franck Duclos; Jane Lee; Roger A Williamson; Kevin P Campbell; Susan V Brooks

doi:10.1152/physiolgenomics.00311.2004

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Muscles of mice deficient in α-sarcoglycan maintain large masses and near control force values throughout the life span

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Muscles of mice deficient in α-sarcoglycan maintain large masses and near control force values throughout the life span

Christina M Consolino, Franck Duclos, Jane Lee, Roger A Williamson, Kevin P Campbell and Susan V Brooks

Physiological genomics, Vol.22(2), pp.244-256

07/14/2005

DOI: 10.1152/physiolgenomics.00311.2004

PMID: 15886330

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Abstract

α-Sarcoglycan-deficient ( Sgca-null) mice provide potential for elucidating the pathogenesis of limb girdle muscular dystrophy type 2D (LGMD 2D) as well as for studying the effectiveness of therapeutic strategies. Skeletal muscles of Sgca-null mice demonstrate an early onset of extensive fiber necrosis, degeneration, and regeneration, but the progression of the pathology and the effects on muscle structure and function throughout the life span are not known. Thus the phenotypic accuracy of the Sgca-null mouse as a model of LGMD 2D has not been fully established. To investigate skeletal muscle structure and function in the absence of α-sarcoglycan throughout the life span, we analyzed extensor digitorum longus and soleus muscles of male and female Sgca-null and wild-type mice at 3, 6, 12, and 18 mo of age. Maximum isometric forces and powers were measured in vitro at 25°C. Also determined were individual myofiber cross-sectional areas and numbers, water content, and the proportion of the cross section occupied by connective tissue. Muscle masses were 40–100% larger for Sgca-null compared with age- and gender-matched wild-type mice, with the majority of the increased muscle mass for Sgca-null mice attributable to greater connective tissue and water contents. Although the greater mass of muscles in Sgca-null mice was primarily noncontractile material, absolute forces and powers were maintained near control levels at all ages, indicating a successful adaptation to the deficiency in α-sarcoglycan not observed at any age in LGMD 2D patients.

Details

Title: Subtitle: Muscles of mice deficient in α-sarcoglycan maintain large masses and near control force values throughout the life span
Creators: Christina M Consolino - Institute of Gerontology and, Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan; and
Franck Duclos - Howard Hughes Medical Institute and
Jane Lee - Howard Hughes Medical Institute and
Roger A Williamson - Department of Obstetrics and Gynecology, University of Iowa, Iowa City, Iowa
Kevin P Campbell - Howard Hughes Medical Institute and
Susan V Brooks - Institute of Gerontology and, Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan; and
Resource Type: Journal article
Publication Details: Physiological genomics, Vol.22(2), pp.244-256
DOI: 10.1152/physiolgenomics.00311.2004
PMID: 15886330
NLM abbreviation: Physiol Genomics
ISSN: 1094-8341
eISSN: 1531-2267
Language: English
Date published: 07/14/2005
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Obstetrics and Gynecology
Record Identifier: 9984020873702771

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