Journal article
Mutation Screening of Candidate Genes in Patients with Nonsyndromic Sagittal Craniosynostosis
Plastic and reconstructive surgery (1963), Vol.137(3), pp.952-961
03/2016
DOI: 10.1097/01.prs.0000479978.75545.ee
PMCID: PMC4770826
PMID: 26910679
Abstract
Craniosynostosis is a condition that includes the premature fusion of one or multiple cranial sutures. Among various craniosynostosis forms, sagittal nonsyndromic craniosynostosis is the most prevalent. Although different gene mutations have been identified in some craniosynostosis syndromes, the cause of sagittal nonsyndromic craniosynostosis remains largely unknown.
To screen for candidate genes for sagittal nonsyndromic craniosynostosis, the authors sequenced DNA of 93 sagittal nonsyndromic craniosynostosis patients from a population-based study conducted in Iowa and New York states. FGFR1-3 mutational hotspots and the entire TWIST1, RAB23, and BMP2 coding regions were screened because of their known roles in human nonsyndromic or syndromic sagittal craniosynostosis, expression patterns, and/or animal model studies.
The authors identified two rare variants in their cohort. A FGFR1 insertion c.730_731insG, which led to a premature stop codon, was predicted to abolish the entire immunoglobulin-like III domain, including the ligand-binding region. A c.439C>G variant was observed in TWIST1 at its highly conserved loop domain in another patient. The patient's mother harbored the same variant and was reported with jaw abnormalities. These two variants were not detected in 116 alleles from unaffected controls or seen in the several databases; however, TWIST1 variant was found in a low frequency of 0.000831 percent in Exome Aggregation Consortium database.
The low mutation detection rate indicates that these genes account for only a small proportion of sagittal nonsyndromic craniosynostosis patients. The authors' results add to the perception that sagittal nonsyndromic craniosynostosis is a complex developmental defect with considerable genetic heterogeneity.
Risk, II.
Details
- Title: Subtitle
- Mutation Screening of Candidate Genes in Patients with Nonsyndromic Sagittal Craniosynostosis
- Creators
- Xiaoqian Ye - New York and Albany, N.Y.; University Park, Pa.; Iowa City, Iowa; Wuhan, People's Republic of China; and Paris and Pessac, France From the Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai; the Congenital Malformations Registry, New York State Department of Health; the Department of Anthropology, Pennsylvania State University; the Department of Epidemiology, University of Iowa; the State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University; the Department of Pediatric Hematology, Robert Debré Hospital; and Université de Bordeaux, UMR5199 PACEA, Bordeaux Archaeological Sciences Cluster of ExcellenceAudrey GuilmatreBoris RevaInga PeterYann HeuzéJoan T RichtsmeierDeborah J FoxRhinda J GoedkenEthylin Wang JabsPaul A Romitti
- Resource Type
- Journal article
- Publication Details
- Plastic and reconstructive surgery (1963), Vol.137(3), pp.952-961
- DOI
- 10.1097/01.prs.0000479978.75545.ee
- PMID
- 26910679
- PMCID
- PMC4770826
- NLM abbreviation
- Plast Reconstr Surg
- ISSN
- 0032-1052
- eISSN
- 1529-4242
- Publisher
- United States
- Grant note
- 5 R01DD000350 / NCBDD CDC HHS 1 P01 HD078233 / NICHD NIH HHS R01 DD000350 / NCBDD CDC HHS P01 HD078233 / NICHD NIH HHS R01 DE022988 / NIDCR NIH HHS
- Language
- English
- Date published
- 03/2016
- Academic Unit
- Epidemiology; Biostatistics
- Record Identifier
- 9983995056902771
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