Journal article
Mutational and gene dosage analysis of calcium-activated potassium channels in Drosophila: Correlation of micro- and macroscopic currents
Neuron (Cambridge, Mass.), Vol.4(2), pp.313-321
1990
DOI: 10.1016/0896-6273(90)90105-O
PMID: 2106331
Abstract
In Drosophila, two Ca
2+-activated K
+ currents, I
CF and I
CS, have previously been distinguished in conventional voltage clamp experiments. The
slowpoke(slo) mutation eliminates IcF specifically. We report that in patch clamp recordings a single-channel Ca
2+-activated K+ current is readily distinguished from other channel activities in normal larval muscle membrane, whereas no such current is observed in slo muscles. This single channel current thus correlates with the macroscopic I
CF. No obvious differences in amplitude or properties were detected between normal (+/+) and heterozygous (
slo/+) I
CF channels in whole-cell voltage clamp recordings or single-channel patch clamp recordings. These results are consistent with the hypothesis that
slo is a structural gene for the IcF channels only under certain conditions. The selective effect of the slo mutation may reflect a defect in a regulatory mechanism that is specific for the functioning of the IcF channel protein.
Details
- Title: Subtitle
- Mutational and gene dosage analysis of calcium-activated potassium channels in Drosophila: Correlation of micro- and macroscopic currents
- Creators
- Akira KomatsuSatpal SinghPerry RatheChun-Fang Wu
- Resource Type
- Journal article
- Publication Details
- Neuron (Cambridge, Mass.), Vol.4(2), pp.313-321
- DOI
- 10.1016/0896-6273(90)90105-O
- PMID
- 2106331
- NLM abbreviation
- Neuron
- ISSN
- 0896-6273
- eISSN
- 1097-4199
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 1990
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984070953502771
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