Mutations in the rpoB Gene of Multidrug-Resistant Mycobacterium tuberculosis Isolates from China

Jun Yue; Wei Shi; Jingping Xie; Yao Li; Erliang Zeng; Honghai Wang

doi:10.1128/JCM.41.5.2209-2212.2003

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Mutations in the rpoB Gene of Multidrug-Resistant Mycobacterium tuberculosis Isolates from China

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Mutations in the rpoB Gene of Multidrug-Resistant Mycobacterium tuberculosis Isolates from China

Jun Yue, Wei Shi, Jingping Xie, Yao Li, Erliang Zeng and Honghai Wang

Journal of clinical microbiology, Vol.41(5), pp.2209-2212

05/2003

DOI: 10.1128/JCM.41.5.2209-2212.2003

PMCID: PMC154692

PMID: 12734282

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Abstract

Mutations in the 81-bp rifampin resistance determining region (RRDR) and mutation V176F locating at the beginning of the ropB gene were analyzed by DNA sequencing of 86 Mycobacterium tuberculosis clinical isolates (72 resistant and 14 sensitive) from different parts of China. Sixty-five mutations of 22 distinct kinds, 21 point mutations, and 1 insertion were found in 65 of 72 resistant isolates. The most common mutations were in codons 531 (41%), 526 (40%), and 516 (4%). Mutations were not found in seven (10%) of the resistant isolates. Six new alleles within the RRDR, along with five novel mutations outside the RRDR, are reported. None of isolates contained the V176 mutation.

Mycobacteriology and Aerobic Actinomycetes

Details

Title: Subtitle: Mutations in the rpoB Gene of Multidrug-Resistant Mycobacterium tuberculosis Isolates from China
Creators: Jun Yue - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University
Wei Shi - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University
Jingping Xie - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University
Yao Li - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University
Erliang Zeng - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University
Honghai Wang - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.41(5), pp.2209-2212
DOI: 10.1128/JCM.41.5.2209-2212.2003
PMID: 12734282
PMCID: PMC154692
NLM abbreviation: J Clin Microbiol
ISSN: 0095-1137
eISSN: 1098-660X
Publisher: American Society for Microbiology
Language: English
Date published: 05/2003
Academic Unit: Preventive and Community Dentistry; Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute; Biostatistics; Dental Research
Record Identifier: 9984065469302771

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