Mycoplasma ovipneumoniae induces inflammatory response in sheep airway epithelial cells via a MyD88-dependent TLR signaling pathway

Di Xue; Yan Ma; Min Li; Yanan Li; Haixia Luo; Xiaoming Liu; Yujiong Wang

doi:10.1016/j.vetimm.2014.11.008

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Mycoplasma ovipneumoniae induces inflammatory response in sheep airway epithelial cells via a MyD88-dependent TLR signaling pathway

Journal article

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Mycoplasma ovipneumoniae induces inflammatory response in sheep airway epithelial cells via a MyD88-dependent TLR signaling pathway

Di Xue, Yan Ma, Min Li, Yanan Li, Haixia Luo, Xiaoming Liu and Yujiong Wang

Veterinary immunology and immunopathology, Vol.163(1-2), pp.57-66

01/15/2015

DOI: 10.1016/j.vetimm.2014.11.008

PMID: 25440083

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Abstract

Mycoplasma ovipneumoniae (M. ovipneumoniae) is a bacterium that specifically infects sheep and goat and causes ovine infectious pleuropneumonia. In an effort to understand the pathogen–host interaction between the M. ovipneumoniae and airway epithelial cells, we investigated the host inflammatory response using a primary air–liquid interface (ALI) epithelial culture model generated from bronchial epithelial cells of Ningxia Tan sheep (Ovis aries). The ALI culture of sheep bronchial epithelial cells showed a fully differentiated epithelium comprising distinct epithelial types, including the basal, ciliated and goblet cells. Exposure of ALI cultures to M. ovipneumoniae led to increased expression of Toll-like receptors (TLRs), and components of the myeloid differentiation factor 88 (MyD88)-dependent TLR signaling pathway, including the MyD88, TNF receptor-associated factor 6 (TRAF6), IL-1 receptor-associated kinases (IRAKs) and nuclear factor-kappa B (NF-κB), as well as subsequent pro-inflammatory cytokines in the epithelial cells. Of interest, infection with M. ovipneumoniae failed to induce the expression of TANK-binding kinase 1 (TBK1), TRAF3 and interferon regulatory factor 3 (IRF3), key components of the MyD88-independent signaling pathway. These results suggest that the MyD88-dependent TLR pathway may play a crucial role in sheep airway epithelial cells in response to M. ovipneumoniae infection, which also indicate that the ALI culture system may be a reliable model for investigating pathogen–host interactions between M. ovipneumoniae and airway epithelial cells.

Airway epithelial cells

Air–liquid interface culture

M. ovipneumoniae

Sheep

Toll-like receptor signaling

Details

Title: Subtitle: Mycoplasma ovipneumoniae induces inflammatory response in sheep airway epithelial cells via a MyD88-dependent TLR signaling pathway
Creators: Di Xue - Ningxia University
Yan Ma - Ningxia University
Min Li - Ningxia University
Yanan Li - Ningxia University
Haixia Luo - Ningxia University
Xiaoming Liu - Ningxia University
Yujiong Wang - Ningxia University
Resource Type: Journal article
Publication Details: Veterinary immunology and immunopathology, Vol.163(1-2), pp.57-66
DOI: 10.1016/j.vetimm.2014.11.008
PMID: 25440083
NLM abbreviation: Vet Immunol Immunopathol
ISSN: 0165-2427
eISSN: 1873-2534
Publisher: Elsevier B.V
Grant note: DOI: 10.13039/501100001809, name: National Natural Science Foundation of China, award: 31460660, 31172278, 31260035
Language: English
Date published: 01/15/2015
Academic Unit: Anatomy and Cell Biology
Record Identifier: 9984288747902771

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