Journal article
Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling-mediated mitochondria pathway
BMC microbiology, Vol.16(1), 222
09/23/2016
DOI: 10.1186/s12866-016-0842-0
PMCID: PMC5035462
PMID: 27663303
Abstract
Background: Mycoplasma ovipneumoniae (M. ovipneumoniae) is a species of Mycoplasma bacteria that specifically infects sheep and goat, causing ovine infectious pleuropneumonia. However, the mechanism underlying the pathogen-host interaction between M. ovipneumoniae and airway epithelial cells is unknown. Methods: A primary air-liquid interface (ALI) epithelial culture model generated from the bronchial epithelial cells of Ningxia Tan sheep (ovis aries) was employed to explore the potential mechanism of M. ovipneumoniae-induced cell apoptosis by characterizing the production of reactive oxygen species (ROS), methane dicarboxylic aldehyde (MDA) and anti-oxidative enzymes, as well as the mitochondrial membrane potentials, cytochrome C release, and activities of ERK and caspase signalling pathways. Results: Increased ROS production and MDA concentration with mitochondrial membrane dysfunction and apoptotic cell death but decreased expression of the antioxidant enzymes catalase (CAT), glutathione synthetase (GSS), total superoxide dismutaes (T-SOD) and Mn-SOD were observed in sheep airway epithelial cells infected with M. ovipneumoniae. Mechanistically, the M. ovipneumoniae-induced cell apoptosis and disruption of mitochondrial integrity reflected mechanisms by which pathogen-activated mitogen-activated protein kinase (MAPK) signalling sequentially led to mitochondrial damage and release of Cyt-C into the cytoplasm, which in turn triggered the activation of caspase signalling cascade, resulting in the apoptosis of host cells. Conclusions: These results suggest that M. ovipneumoniae-induced ROS and MAPK signalling-mediated mitochondrial apoptotic pathways might play key roles in the pathogenesis of M. ovipneumoniae infection in sheep lungs.
Details
- Title: Subtitle
- Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling-mediated mitochondria pathway
- Creators
- Yanan Li - Ningxia UniversityZhongjia Jiang - Ningxia UniversityDi Xue - Ningxia UniversityGuangcun Deng - Ningxia UniversityMin Li - Ningxia UniversityXiaoming Liu - Ningxia UniversityYujiong Wang - Ningxia University
- Resource Type
- Journal article
- Publication Details
- BMC microbiology, Vol.16(1), 222
- Publisher
- BioMed Central
- DOI
- 10.1186/s12866-016-0842-0
- PMID
- 27663303
- PMCID
- PMC5035462
- ISSN
- 1471-2180
- eISSN
- 1471-2180
- Grant note
- 31572494; 31460660 / ; 2012BAD12B07 / ; 2012CB518801 / ;
- Language
- English
- Date published
- 09/23/2016
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984288751602771
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